Is FBS sufficient for papain inactivation? Why/Why not? Do I really need to use Ovomucoid in my Papain Stop Solution?

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Characterization of exosomes?

I have started to work with isolation of exosomes and I used the protocol from this paper (ultracentrifuge): "Isolation and characterization of exosomes from cell culture supernatants and...

05 June 2018 8,882 2 View

What is the minimum amount of exosomes to enrich a culture medium?

I want to enrich a culture medium with isolated exosomes of a given cell line for cell signaling studies in another kind of cells. What is the minimum amount of exosomes to enrich this culture medium?

04 May 2018 2,673 3 View

(Western Blotting/ SDS PAGE / Proteomics) should the sample preparation for studying membrane proteins be different for preparing cytosolic proteins?

I'm trying to find out which membrane protein (~ 30kDa) my samples of mesenchymal stem cells are expressing. I made a SDS-PAGE and the gel was stained with Coomassie Blue. The band in question is...

05 June 2016 9,280 3 View

Does anyone here work with intestinal cell primary cultures?

Intestinal epithelial organoids

07 August 2013 8,064 11 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

fatal error

16 June 2021 0 0 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View