Is FBS sufficient for papain inactivation? Why/Why not? Do I really need to use Ovomucoid in my Papain Stop Solution?

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Characterization of exosomes?

I have started to work with isolation of exosomes and I used the protocol from this paper (ultracentrifuge): "Isolation and characterization of exosomes from cell culture supernatants and...

05 June 2018 9,033 2 View

What is the minimum amount of exosomes to enrich a culture medium?

I want to enrich a culture medium with isolated exosomes of a given cell line for cell signaling studies in another kind of cells. What is the minimum amount of exosomes to enrich this culture medium?

04 May 2018 2,791 3 View

(Western Blotting/ SDS PAGE / Proteomics) should the sample preparation for studying membrane proteins be different for preparing cytosolic proteins?

I'm trying to find out which membrane protein (~ 30kDa) my samples of mesenchymal stem cells are expressing. I made a SDS-PAGE and the gel was stained with Coomassie Blue. The band in question is...

05 June 2016 9,415 3 View

Does anyone here work with intestinal cell primary cultures?

Intestinal epithelial organoids

07 August 2013 8,174 11 View

An animal in not a rock (e.g., Koch 2013), it has an internal constitution that can be modified?

Vertebrates and invertebrates (e.g., arthropods: insects, crustaceans, etc.) have two different neuro-muscular systems given their differential construction: the former have an endoskeleton and...

07 August 2024 648 0 View

Do I need specific antibodies to stain for a fusion protein?

I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...

07 August 2024 5,338 2 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Are the GABAergic neurons electrophysiologically equal in all brain areas?

Firing in GABAergic neurons has been characterized in several areas of the brain, but patterns are the same?

04 August 2024 4,090 0 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View