I want to perform some biochemical experiments(cancer effect of ibuprofen on the liver) on ibuprofen, but these experiments require dissolve ibuprofen in a solvent for injection it to the rats. The ibuprofen not dissolved in water, 0.9%NaCl, DMSO, but dissolve in ethanol. Is ethanol a suitable solvent for injection?
Hi Hossam,
As ibuprofen structurally contains both hydrophilic and hydrophobic functional groups it is not straightforward to dissolve in water. Ethanol as a solvent is no problem externally but it may have different affect on the liver function (as ethanol is absorbed by the liver) if used as absolute ethanol (high concentration) - so I would recommend dilution to the point where ethanol is just enough to dissolve the ibuprofen but water content is higher for injection to ascertain ibuprofen effect.
Vidhura Mahendra
Thank you, In a puplished paper, the solvent used is 0.9%NaCl but ibuprofen not dissolve in NaCl ,my question, How inject the rats in this paper using NaCl??
The paper link:http://clincancerres.aacrjournals.org/content/11/4/1618.full.pdf+html
Hossam,
The most important factor here is the 0.9% of NaCl which is commonly known as the Saline water. So you can see the NaCl concentration is very weak.
So if your question is related to the solubility in which you find it is not soluble in 0.9% NaCl you may use dilute ethanol as I mentioned before.
Hope that helps.
Hydrotropic agents have been used experimentally to enhance aqueous solubility of hydrophobic drugs. In one study, the aqueous solubility of ibuprofen was found to increase tremendously by mixing with a hydrotropic agent like sodium benzoate at a concentration of 1M.So, if ethanol, which is a good solvent for ibuprofen is considered toxic to the liver, try the use of sodium benzoate to increase the aqueous solubility of ibuprofen.
Dear Davies. Good suggestion. Could you send the citation of the work which used sodium benzoate to dilute ibuprofen in water. Thank you in advance .
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