6 Questions 20 Answers 0 Followers
Questions related from Sina Ben Ali
I am looking for a method that enables Bacillus Cereus enterotoxin detection in human stool samples without prior cultivation. Direct detection that proves that enterotoxins were already produced...
04 March 2021 749 1 View
Hello everyone, I have performed amplicon sequencing (MiSeq, Illumina) of a 180 bp region in transgenic Zea mays. The target region is part of the tansgene. I have detected a SNP at the same...
09 August 2017 753 28 View
Hallo experts, I perform gene expression analysis using RT qPCR. I compare two groups of maize kernels (group A and B). I have four pools per group and every pool consists of ten different maize...
17 March 2017 8,419 3 View
Hello, I plan to perform methylation-sensitive HRM analysis of maize samples. 1. Are methylation-sensitive primer pairs enough or is it necessary to design both, primer pairs for the methylated as...
03 February 2017 7,980 1 View
My greenhouse maize plants are too thin. They have strips on the leaves. The conditions are 14h day/10h night; 26-28°C day and 20-22°C night; 60% humidity; peat soil mixed with little sand. I use...
23 February 2015 5,931 6 View
I have cloned amplified PCR fragments of a genomic maize sample into plasmids. I obtained six plasmids containing the same region. The genomic maize sample belonged to a single maize grain. The...
01 October 2014 631 11 View
