There are many methods to evaluating antioxidant activity of compound. Why DPPH assay is used often?
https://www.sciencedirect.com/topics/medicine-and-dentistry/dpph-radical-scavenging-assay
2,2-Diphenyl-1-picrylhydrazyl (DPPH) radical scavenging assay. DPPH radical scavenging assay is among the most frequently used methods and offers the first approach for evaluating antioxidant activity. It is an ET-based method with HAT mechanism being only a marginal reaction pathway in the assay. https://www.sciencedirect.com/science/article/pii/S1756464615000511
FRAP assay can also be used. FRAP assay stands for Ferric Reducing Antioxidant Power Assay. ... The assay measures the antioxidant potential in samples through the reduction of ferric iron (Fe3+) to ferrous iron (Fe2+) by antioxidants present in the samples. https://www.abcam.com/frap-assay-kit-ferric-reducing-antioxidant-power-assay-ab234626.html
Phosphomolybdeum assay can also be used. % of inhibition = control OD - sample OD/ Control OD X 100. Its not only phosphomolybdeum assay gives you the result of total antioxidant capacity for your plant. You can try for ABTS method also. this assay also gives the total antioxidant capacity. https://www.researchgate.net/post/How_to_calculate_total_antioxidant_capacity_in_plants_from_Phosphomolybdenum_assay_from_raw_optical_density_value10
simple answer is no. You can choose a lot of other methods including ABTS, DMPD for scavenging assay. In addition, you may choose FRAP, Phosphomolybdenum blue, reducing power, and many more. You may read my one paper.
Article Comparative Bio-Active Compounds Determination and In Vitro ...
The best does not exist. Each assay has its own problems. From my pint of view, DPPH ( as well as FRAP and phosphomolybdeium) are among the worst.
I agree with Yurii V Geletii. Each assay has its own uniqueness
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