I am performing a minimum inhibitory concentration (MIC) test using the broth microdilution method. The compounds I am testing are polar which means I could dissolve my compounds in distilled water to make a stock solution for serial dilution later. However, I am worried about possible contamination with environmental bacteria which may cause problems in my MIC testing later.
Could anyone advise whether contamination would be an issue if I used distilled water as a solvent? Would DMSO be a better solvent as I've heard that DMSO is toxic to bacteria at high concentrations which would kill any environmental bacteria that happened to be in my stock solution.
(note: I cannot autoclave my compounds as they are powder in nature and they may degrade at temperatures below 121 degrees Celsius.)
Is there any reason you can't just filter sterilize your distilled water stocks of your compounds?
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