Greetings,
I am trying to measure L-lactate in mice blood plasma. For that, Is it absolutely necessary to remove protein form plasma sample before measuring lactate?. How much does this affect the result if proteins are not removed?.
Can somebody please enlighten me?.
Regards,
Seema
Sorry, I do plasma physics like the sun. I get these medical questions all the time and do not know how to end them.
Dick
Hello Seema Thappa: The values of ammonia concentration were higher in the plasma specimens compared with the deproteinization ones . Deproteinization is necessary for accurate measurement of ammonia using GLDH assay in the blood plasma of subjects with liver injury. Blood lactate concentration ([La−] b) is one of the most often measured parameters during clinical exercise testing as well as during performance testing of athletes.[Lai may be indicative of ischemia or hypoxemia. The following links would help:
https://academic.oup.com/labmed/article/48/4/339/4372133
Article Blood Lactate Measurements and Analysis during Exercise: A G...
https://www.promega.com/-/media/files/resources/protocols/technical-manuals/101/lactate-glo-assay-protocol.pdf?la=en
Regards--
