I am trying to define CSF1R expressing cell populations in human tissues, and CD68 was recommended as a pan-macrophage marker, but in co-staining studies I have found that CD68+ cells are only a subset of the CSF1R positive population. I have found several publications in literature that both support and contradict this. Any thoughts?
Thanks!
PS: the vendor has not extensively characterized the CSF1R antibody (peptide antigen), but based on sequence homology does not anticipate any cross-reactivity even with closest homologs.
Dear Amit,
CD68 is a pan macrophage marker but expresses mostly in tissue macrophages or histiocytes. If your target macrophages are from other tissues, you might have problem. I can suggest, that you can check CD163 which is also a validated marker of tissue macrophages.
Good luck
well CD68 can also be expressed by neutrophils and monocytes too. So, it's not exclusive only to macrophages. The way to know if it's actually a macrophage is through surface staining. CD68 is expressed on the surface of macrophages while it is mostly intracellular for other cell types. Also, it's expression depends on levels of differentiation, mostly associated cwith tissue-resident macrophages (as mentioned above)
- Badges
- Science topic
- Similar topics
- Biological Science
- Immunology
- Antibodies