I have seen different results published online using CATALASE. I know that the outcome of the analysis depends on the type of tissue, and protocol used but I have seen gotten different results from my brain tissue sample using sample protocol.
Please note that if you want check OXIDATIVE STRESS, you can not stick to the enzymes. You should measure lipid peroxidation, protein carbonyl, GHS:GSSG ratio. They indicate if oxidative stress occured or not.
However CAT results are somehow strange in in vivo conditions.
yes It's a reliable parameters , you can also use another parameters of OS such as antioxidants , lipid peroxidation , DNA damage, etc.
If you consider that catalase is part of antioxidative defense and plays a role in the conversion of H2O2 to H2O and O2 I would say it is a good oxidative stress marker.
The scientific literature provides evidence that an increase or decrease in catalase activity/expression (as well as superoxide dismutase (SOD), glutathione reductase (GSR), glutathione transferase (GST), selenoprotein glutathione peroxidase 4 (GPX4) and many others) may indicate an oxidative damage.
Studies show that catalase enzymatic activity may increase and catalase gene expression may decrease in the same tissue, or they may increase in one tissue and decrease in another.
For example, there are molecules that are part of the antioxidative defense that increase in the brain and decrease in the gonads in relation to reproductive cycle, showing how complex their action can be.
Thus, I would say that catalase is definitely a marker of oxidative stress especially if it is accompanied by other markers studied at transcriptomic and proteomic level, such as those mentioned above.
in my agronomic study , the response of CAT and glutathione proxidase to Ti was simillar to dityrosine and 8-OH-DGresponse. however, the response of SOD to Ti was different with dityrosine and 8-OH-DGresponse. it may conclude that enzymes values must be compared with the dityrosine and 8-OH-DGvalues- it may help to judge about the results of a special enzymes
please see my paper in MAYDICA journal,
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