Is APH assay reliable and better than MTT in determining cell viability?

More Behin Sundara Raj's questions See All

What is the protocol for liposome preparation for the essentail oil? and what things we was focus during the preparation?

liposome, essentail oi

19 May 2024 4,918 1 View

Is there any possibility to download the research paper citation from researchgate database?

Dear Prof, Greetings ..... I'm Sebastian, would like to do research on paper publication and its impact in the ResearchGate database, like Web of Science and Scopus. So if you all have any idea...

15 April 2024 7,244 1 View

How do i calculate cut off values in ELISA?

I am running ELISA for different biomarkers against meningitis. I need to know how to calculate cutoff values for it in ELISA. There is no negative control or positive control for these kits,...

07 April 2024 3,079 1 View

Is it possible to enhance the electrical conductivity in Carbon Fiber Reinforced Plastics?

Can we metallize CFRP by adding Copper or Silver nano particles to enhance the electrical conductivity? or any other suggestions?

23 March 2024 7,364 2 View

What are the testing methods to verify Electrical conductivity in Carbon Fiber Reinforced Plastics?

Testing methods to determine how well electrical current can pass through the manufactured CFRP composite laminates

23 March 2024 8,846 3 View

What is the procedure for the anti inflammatory studies using essential oil ( nitric oxide) ? or any other anti inflammatory studies?

Essential oil anti inflammatory nitric oxide

17 March 2024 5,036 2 View

Can any one give Special properties and examples of Graphs that are (i) Uniquely (ii) doubly (iii) triply k- dimensional?

Let M = {v1, v2 ... vn} be an ordered set of minimum number of vertices in a graph G. Then the vector of distances (d(u, v1), d(u, v2) ... d(u, vn)) is called the M -coordinates of a vertex u of...

24 February 2024 3,228 2 View

How to calculate ic 50 value in gram ( essential oil)?

Essential oil gram ic 50 calculate

15 February 2024 9,767 1 View

How to convert the essential oil miro litre into micro gram?

Essential oil, micro gram , micro litre

10 February 2024 8,218 1 View

What is different of crude oil, essential oil and oil?

Different of crude oil essential oil and oil extract form the plant

09 February 2024 5,179 0 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

The aqueous fraction of the hydroethanolic extract is showing the presence of palmitic acid. What is the mechanism responsible ?

Palmitic acid presence in aqueous fraction

05 August 2024 8,624 4 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Yuriy Fedorov

I can recommend Resazurin assay instead of either one in question: http://en.wikipedia.org/wiki/Resazurin. Works great for all 3 cell lines.

Carl Manner

I've used MTT for proliferation studies in those cell lines, and it works well. For viability I always used trypan blue and flow cytometry - it's nearly foolproof (and inexpensive)