Carl Manner

6 Questions 18 Answers 0 Followers

Questions related from Carl Manner

Why are my cells dying in MLR?

Our lab recently did a test run mixed lymphocyte reaction (MLR) using normal human PBMCs. Medium composition was HyClone RPMI-1640 +10%hiFBS +P/S +Fungizone +2.05 mM L-glutamine. The feeder cells...

28 October 2016 8,748 3 View

What is a good marker for Th3 cells in multicolor flow panels?

I'm looking for a marker specific to T helper 3 cells (Th3) for use in a multicolor flow cytometry panel. Any suggestions for a specific marker, or combination of markers, is greatly appreciated.

18 September 2016 3,225 2 View

Why are these protein isoforms conserved in vertebrates?

I study two protein isoforms of WNT5A that differ from one another by only 18 amino acids post-processing. These isoforms are highly conserved among vertebrates. We see no evidence that these...

28 March 2016 421 2 View

Under what conditions should I exclude a Western blot (of total and phosphoproteins) in the analysis of signaling pathway activation?

I've been blotting samples derived from cells that were treated with a ligand. I expect this ligand to activate a signaling pathway, and I expect certain components of this pathway to be...

22 February 2016 1,484 4 View

How can I determine tissue/disease state specific expression of protein isoforms?

A question from my PI:"We would like to search a RNA-seq database(s) using a sequence to determine what tissues, cells etc a particular transcript is expressed in. I looked on the NCBI and UCSC...

14 December 2015 2,143 3 View

What could cause a negative band in WB?

I'm blotting against STAT3 and pSTAT3Y710. My pSTAT3 antibody works great, but I observed a negative band (a white band that is lower intensity than background) at the predicted MW for STAT3 when...

02 November 2015 6,310 6 View