i am trying to work on efflux pump systems such ac AcrAB, kexd and others in my Msc project. i cant find enough information about PCR protocols. i am looking for to some help for my project.
thnx for reply
Have a nice day everyone, I'm stuck in extracting datasets out of MPI-ESM-MR netcdf files because the latitude ranges from -3*10^6 to 3*10^6 and longitude ranges -5,9*10^6 to 5,9*10^6. Plus, the...
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Of course, there are verity of material, but i need essential material and buffers for this technique. maybe there are some condition for reagent selection that i dont know them. Thank you!
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I am measuring the crop water use (ET) by using pots and lettuce crop as plant material, all the data of daily weighted pots are ready but I want to find the surface area of each pot. Can you...
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I am measuring the crop water use (ET) by using pots and lettuce crop as plant material, all the data of daily weighted pots are ready but I want to find the surface area of each pot. Can please...
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I'm making reprogramming of MEFs experiment and I used Piggy bac, Sleeping beauty and episomal transposition systems. After electroporation i froze the cells, then i thawed the cells but there...
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Do you think that genetic inheritance or the environment is more influential in the development of substance abuse ?
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Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...
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Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...
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I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
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Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...
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Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...
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Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...
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Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...
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Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...
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My purpose is to recognize the volatility clustering property without statistical tests such as The Lagrange Multiplier (LM) test.
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