LEDs on Vis Light photo degradation of MB water sol via N doped TiO2 in a mini flume?

Good day to all, I have no experience with light emitting diodes. I am building a glass mini flume to demonstrate the photo degradation of Methylene Blue in water running over self cleaning...

26 February 2021 4,026 5 View

How to increase the lower detection limit of ELISA?

Hi all, I am trying to establish an ELISA method for the quantification of a recombinant protein injected into mice. In the meantime I am trying to make calibration curves with the purified...

27 January 2021 8,702 3 View

Best glass slides for metal oxide UV-vis and PL measurements?

Hi there, I am currently using Alfa Aesar fused quartz slides for my UV-vis and PL measurements of TiO2, but it seems that their slides emit in the UV around the same wavelength region where I...

19 January 2021 438 3 View

What is the best and most efficient way to conduct a meta analysis?

I have never conducted a meta analysis before, so I was wondering how to approach it. Let's say I wanted to look a how a specific metric has evolved over the past 20 years, for example the...

08 November 2020 8,663 2 View

Longest duration for an experiment involving oral gavage?

Hi, I was wondering what the longest period of time anyone has done an experiment involving daily oral gavage? I have a model that takes 6 months to fully develop and need to begin treatment at...

05 November 2020 6,243 3 View

What organic solvent is silver (I) sulfide not soluble in, and how to unambiguously confirm presence of P-N double bonds?

This is really two questions. I'm trying to convert P-S bonds into P-N bonds using Ag+ to pull off the sulfur, but when attempting the reaction in THF, either the silver sulfide was dissolved in...

17 June 2020 9,066 8 View

Sampling rate in matlab?

Can I know what is the sampling rate of ifft() command which is a build in function in matlab?

01 June 2020 7,937 12 View

Finding Hidden Defect in ceramic sample in matlab?

I am dealing with ' Optimal signal processing of frequency-stepped CW radar data' from the website of Optimal Signal Processing of Frequency-Stepped CW Radar Data I want to find the hidden...

27 May 2020 1,125 12 View

Can Coronaviruses quorum sense?

Looking at the various CoV genomes, there are peptides/small proteins, aside from ORF1/2 on the genome, of which functions are unknown. These could circulate and in theory, once in abundance,...

25 March 2020 5,948 5 View

Where can I buy photodiodes?

Hi there, I'm looking to buy various photodiodes/solar cells for some experiments. The cells I'm looking for are InGaP, CZAS (and CZT) and amorphous silicon. Any pointers to where I could buy...

13 February 2020 2,938 5 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

What is the minimum number of exosomes/ml to have a good yield of total RNA for Illumina sequencing?

I am using IZON qEV2 columns to isolate exosomes from 100 ml of cancer cell conditioned medium. in NTA analysis I usually see concentration of 2x10^9 particles/ml. But when I proceed with this...

20 February 2021 6,879 3 View

Is it possible to remove the cells from a tissue and keep only the extracellular matrix for proteomics?

Hello! I would like to know if there is a way to flush/remove all the cells out of a tissue and keep only the extracellular matrix, in order to analyse its composition using e.g. proteomics....

14 February 2021 1,602 4 View

Meniscus formation matrigel?

Hey guys, I am currently doing tube formation assay on matrigel coated coverslips, and i wonder if there is any possibility to get rid of the meniscus formation? I have only a small part of the...

09 February 2021 6,979 3 View

Scanning Electron Microscopy for Extracellular Vesicles?

Can anyone provide or recommend a detailed protocol for preparing extracellular vesicles for SEM? Most protocols that I have found that list the necessary reagents and individual steps are for...

02 February 2021 4,475 3 View

Has anyone used Isolate II RNA mini kit for isolation miRNA from exosomes?

I will be isolating exosomes from plasma with Total Exosome Isolation Kit (Invitrogen). Will Isolate II RNA mini kit (Bioline) be appropriate for miRNA isolation from obtained exosomes? The...

26 November 2020 9,503 5 View

Which ABs concentration for EVs immuno-staining?

Hi all, I have to do a phenotyping study on cell culture derived EVs, in particular I should stain them using primary labelled ABs anti-EVs markers before proceeding to analyze them through...

25 November 2020 4,601 1 View

Can someone please share how to add a spherical wrap / correction to a visual stimulus presented to mice using python?

Hi all I use psychopy 3 to show mice a visual stimulus on an LCD screen while preforming acute extracellular recordings in vivo. I understand that one should use a spherical wrap to the stimulus...

22 November 2020 7,905 2 View

What is the best RNA isolation method from exosomes of serum samples ?

12 November 2020 9,331 7 View

Recommend equipment of in vivo recording in mice brain?

Hello, I am trying to set up an in vivo recording system in mice brain. Which company do you recommend? Neuralynx? TDT? NeuroNexus? Which one do you guys use and why? Please let me know. Thank you!

12 November 2020 2,508 1 View