I need MCF-7 cell lines as a control for my experiments in Peshawar. Does anyone have these in a lab in Pakistan that we can get hold of?
Sorry ..... This cell line is not available here.......
We classify the literature in old or antient and modern literature, my question is that on which ground we do so. what the standard and gauge for that.
19 April 2024 2,168 4 View
"Can anyone recommend recent research papers on fabricated electochemical anodes for water splitting? I am specifically interested in synthesis, characterization, and performance evaluation. Thank...
04 April 2024 237 1 View
Hi everyone, Are there any other alternatives other than the MODELLER program that I can use to complete the missing residues in the crystal structures before running the simulations? We can...
18 December 2023 10,036 3 View
In my clinical observations, I absolutely think that extraction of teeth during pelvic infection can cause an existing pelvic infection to be excecerbated. That's why suitable antibiotic coverage...
09 November 2023 2,690 2 View
I have tried different medium complements for each cell lines but there is a contamination that i don't know. So i couldn't expand them in culture. Which supplement i should use for each cell...
05 November 2023 3,433 3 View
While trying to run the MD simulation with the CHARMM-GUI input file, I encounter such an error during the equilibration phase. How to overcome this? gmx mdrun -v -deffnm ${istep} Program:...
25 May 2023 9,955 4 View
How can I perform ligand-protein interaction analysis (L-P contact) using Gromacs .gro and .xtc outputs on SID (simulation interaction diagram) panel in Schrödinger Maestro version 2022.4? After...
21 March 2023 3,277 0 View
I am trying to calculate protein-ligand contacts (especially hydrophobic interactions) in the allosteric site of a GPCR. Thus, could you suggest a tool that I can use free of charge, except...
26 February 2023 8,160 4 View
I want to use cpptraj for some analysis but I need to convert my topology and trajectory files from Gromacs xtc, tpr format to Amber nc and parm formats. Could you please let me know how can I...
25 February 2023 3,042 2 View
Hi everyone, I am trying to calculate MM-PBSA or MM-GBSA for ligand-protein structures in my simulations with GROMACS 2020.5. version. There are recommended calculations for older versions of...
22 February 2023 7,979 4 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View