I am preparing neocytes concentrates by conventional method i.e. centrifugation and separation method
Congratulations for the noble method involved. I hope your institution would continue to do this so that life of Thalassemic children can be prolonged.
This method of yours is most suitable and we in Himachal are trying to impress upon authorities in blood banks to adopt the same.
Waiting for you to have larger sample for sufficient evidence.
Regards,
Omesh Bharti
Thank you very much for your interest. We are doing it on regular basis to prolong the transfusion interval of thalassemia Patients. It also helpful in reducing the iron over load too.
Please if you could document this in a large sample so that it can be followed in there parts of the country. Is this practice common outside India.
Regards,
Omesh Bharti
Dear Sir
Omesh Kumar Bharti
My one paper is published on the subject i.e.https://www.researchgate.net/file.PostFileLoader.html?id=592567e4b0366d5a18531df6&assetKey=AS%3A497495811211264%401495623652328
Further work is not yet documented. We have done more than 500 neocytes transfusion , results are very useful and have a definite protocol for that in our Lab. We can provide you the rough data, protocol and indications to your mail , if you provide us the same . Two workers out side the India prepared neocytes for transfusion by aphaeresis procedure.
Please send me detailed protocol so that we replicate the same in Himachal.
Regards,
OMESH
Dear Sir,
Please provide me the email ID for further communication
Dear omesh,
summery of raw data conveyed to you by e- mail.
Thanks
Dear Omesh
There is few references of neocytes preparations and transfusion :
1. Montoya AF (1993) Neocyte transfusion: a current perspective. Transfusion Sciences 14(2):147–156
2. Bertholey F, Saint Paul E et al (1990) Transfusion of concentrates enriched in neocytes in thalassemic child, preliminary results. Rev Fr Transfusion Hemobiol 33(3):165–174
3. Kalinin NN, Varlamora SV et al (1991) Neocytapheresis in donors. Sov Med 5:41–43 Klein HG (1982) Transfusion with young erythrocytes (neocytes) in sickle cell anemia. Am J Pediatr Hematol Oncol 4(2):162–165
4. Spanos T, Ladis V et al (1996) The impact of neocyte transfusion in the management of thalassemia Vox. Sang 70(4):217–223
5. Collins AF, Goncalves Dias C, Haddad S et al (1994) Comparison of a transfusion preparation of newly formed red cells and standard washed red cell transfusion in patients with homozygous beta Thalassemia. Transfusion 34:517–520
There are two methods to procure neocytes
1. Neocytapheresis by apheresis method
2. By conventional method of centrifugation and separation method
First method is expensive one as far as Indian scenario is concern .
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