Mireia Rovira

2 Questions 8 Answers 0 Followers

Questions related from Mireia Rovira

Does anyone know a sarcolemma antibody marker (i.e. dystrophin, DAG, sarcoglycan, etc) suitable for zebrafish and fixed-paraffin sections?

Hi there, I have seen that MANDRA1 clone works well in zebrafish but unfortunately the epitope is sensible to fixed and paraffin-embedded samples (as specified by the antibodies suppliers). Does...

11 November 2016 5,496 2 View

How to avoid breaking of muscle cells on cryosections?

I work with fish muscle pieces of about 4x2x3 mm3 and I will need to cut at 10um. I have prepared some blocks of tissue following the standard procedure: cooled isopentane in liquid nitrogen to...

09 September 2014 8,334 3 View