Maulasri Bhatta

3 Questions 6 Answers 0 Followers

Questions related from Maulasri Bhatta

Flow Cytometry vs Western Blotting: not able to get the same result. Why?

Hello, I performed flow cytometry using antibody Pacific Blue™ anti-mouse/rat CD29 Antibody (cat no. 102224, biolegend) in endothelial progenitor cells. Also, in the same cells I performed...

02 February 2018 1,968 3 View

Is anyone familiar with RNA extraction?

I got 260/280 ratio between 1.8-1.99, Also I got 260/230 ratio around 0.6-0.95 and concentration is around 390-400 ng/ul (RNA dissolved in DNAase/RNAase free water). I want to run rtPCR and...

09 September 2014 1,610 4 View

Unable to passage Endothelial progenitor cells.

I have isolated Endothelial progenitor cells from mice bone marrow and grown them for 15-20 days on fibronectin. After 16 days, I need to passage them as to use the cells for FACS or re-seed them....

07 July 2013 589 21 View