Why is my primer amplifying multiple products despite specificity, yet resolved with SNaPshot?

I have several COMT SNPs which I designed primers for using BatchPrimer3. I did all my checks and did a BLAST for all primers to ensure it binds to the correct region. All primers have worked...

14 September 2020 4,143 4 View

ABC elite kit - should I agitate while incubating tissue with ABC?

I inherited a staining protocol from another lab. It specifically says not to swirl the ABC mixture, but instead to let it sit for 30 minutes before adding the tissue. It does not specify if the...

26 January 2020 5,868 2 View

Is there any kind of interference between wireless networks?

during my day to day I could appreciate how the intensity of the wifi networks are altered because of the existence of other networks, here is my question because I want to know if. Is there any...

05 July 2019 5,007 5 View

Plasmid Problems: Is it possible to use RE to cut a section out of a plasmid & immediately amplify it?

I have a 1.6kb insert that has been cloned into a plasmid. I would like to cut a section out of this plasmid that is flanked by restriction sites for ECOR1. Would it be possible to digest the...

11 February 2019 2,848 7 View

Convert a 3D direction vector to yaw and pitch?

I am trying to convert 3D direction vectors (see example) to yaw and pitch (apparently you can't get roll from the vectors). My ultimate goal is to determine the Euler angles from my answers....

09 September 2018 1,472 1 View

Using the gromacs md run -rerun command, is it possible to generate bonded terms?

Hi, I ran some MD simulations in GROMACS and am trying to get a sense of the changes in potential energy of the active site between conditions (with and without inhibitor present). I have...

25 April 2018 7,688 5 View

Why the sewage water I treated using electrocoagulation & iron electrodes resulted to have a very intense yellow color and unstable sludge?

I've already adjusted the pH of my sewage sample between 2-12 but still the result is a water with rust like color. I've already considered changing the current supply and electrolysis time but...

06 April 2018 6,464 3 View

Running MD simulations on small molecules and generating parameters ?

I am generating small molecule parameters using PRODRG and ABT. Why exactly does PRODRG take less time then ABT? I want to know this because I am trying to decide if it is worth it to wait for ABT...

03 April 2018 5,705 2 View

What are the challenges of Regenerating/reviving a dead cell, using bioelectronics or other methods.

dormant cells don't undergo any metabolic processes, but an appropriate stimuli can activate the Partially dead cells, to fully functional cells. Regenerating/reviving a dead cell, using...

10 July 2017 8,810 1 View

Can you detect secreted ATP by luminescence if your supernatans contains FBS?

26 April 2017 3,760 5 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Why is the sum of the elements in a row in a Q matrix equal to zero?

When explaining substitution models, the substitutions are expressed as Q matrix. Why is the sum of the elements in a row zero?

28 February 2021 3,864 3 View

Does different dilution factors affect negatively on NH3 reading ?

I have a sample of treated water sample & asked to obtain ammonia conc. in sample using a machine with 5-25 ppm detection range. However, upon testing with different dilution factors, all...

27 February 2021 8,483 3 View

LEDs on Vis Light photo degradation of MB water sol via N doped TiO2 in a mini flume?

Good day to all, I have no experience with light emitting diodes. I am building a glass mini flume to demonstrate the photo degradation of Methylene Blue in water running over self cleaning...

26 February 2021 4,026 5 View

To date, according to health authorities, 30% of the population's diseases are initiated by drinking water pollution. Do you know this problem?

The main studies are as follows: * regular and timely receipt of information about the state of the environment using automated systems (stationary and mobile); * operational analysis of the...

23 February 2021 912 5 View

How to best wash a 96-well plate containing microplastic?

I am planning my protocol for my dissertation for an experiment quantifying bacterial (E. coli) biofilm formation on micro-plastic (5mm discs of polyethylene bags) by conducting a crystal violet...

23 February 2021 7,868 2 View

Multinomial probabilities and confidence intervals - obtaining the CI of the reference category?

Hi, Some background to my question: I have fitted 1st order multinomial Markov models with covariates. Since all probabiilties (for each row of the transition matrix) sum to 1, I estimate only...

22 February 2021 2,541 4 View

How can I improve the concentration of banana fruits extracted RNA?

Currently, I'm working with banana fruits. I've tried to do RNA extractions with different methods, and I've gotten total RNA without contamination, but I couldn't get a high concentration of...

22 February 2021 6,126 2 View