I am trying to validate AATCC 147 but I get different results everytime I run the test, sometimes I get growth under the fabric sample with samples that have shown a halo in previous runs.
Any tips?
This is a qualitative method but the results should be reproducible. You need to make sure that the sample is in contact with the inoculated agar surface. If the treated fabric is hydrophobic, they may not be in contact with the agar surface. You can soak the fabric in a solution containing few drops of wetting agent before placing it on the inoculated agar surface. You will need to make sure that the wetting agent does not affect the growth of bacteria. You also need to make sure that the agar surafce does not become dry. For quantitative results, you should use AATCC Test Method 100 if you antibacterial agent is leaching type.
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