In order to study the role of histone methyltransferases in blood vessels, we want to do Carotid Artery injury in GM mouse. We don't have any experience so we would welcome any advice on this.
Not really, but you might try checking if there has been any studies done on hypertensive mice or rats or Zucker rats.
I am trying the common carotid artery cannulation in mice but it seems complicated, any suggestions please?
I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...
24 February 2021 8,325 1 View
I've been working with qPCR for some months now. I amplify 2 genes (PSEN2 and HN1), and the protocols are optimized for both and have been working fine. The other day I amplified HN1 for some...
21 February 2021 3,437 3 View
If ethanol has analgesic properties, why isn't it used for clinical purposes?
18 February 2021 8,227 4 View
Hi everyone! I am about to extract RNA for RNA sequencing assays and my Invitrogen kit/protocol...
14 February 2021 1,755 3 View
Hi everyone We need Fast Green to electroporate plasmid into mice embryos, therefore we simply need it to see some color in the liquid we are electroporating so that electroporation is more...
07 February 2021 1,671 2 View
Hello I'm using image registration over the bands of the micasense red-edge camera. Using RedEdge band as the fixed image. After removing inliers I can notice that inliers of the NIR band are...
13 January 2021 3,980 2 View
11 January 2021 3,284 3 View
06 January 2021 1,718 2 View
Somewhere in papers, it is written as Mn 2p3/2 XPS spectra can be deconvoluted into four peaks at 640.83, 641.92, 642.97 and 644.56 eV assigned to Mn(II), Mn(III), Mn(IV), and the shakeup peak,...
24 December 2020 4,323 4 View
16 December 2020 9,657 5 View
Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...
02 March 2021 4,569 4 View
Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...
02 March 2021 6,865 1 View
The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....
27 February 2021 4,450 2 View
In R-studio, there are many commands of Gumbel package. Arguments are also different. I`m asking about the alpha parameter of the Copula which must be greater than 1. If this is the one used to...
25 February 2021 5,229 3 View
Hello, I asm currently working with alginate and silk fibroin hydrogels. I prepared a blend of alginate and aqueous silk fibroin (straight from dialysis) and resorted to ionic crosslinking to...
23 February 2021 8,249 2 View
I want to map these river geoforms with as little subjectivity as possible. I have a high resolution DEM generated by drone data.
22 February 2021 7,800 3 View
I am currently working on gene clustering based on co-expression pattern in mouse brain. The problem is I do not have some solid way to test my result. Are there any suggestions for databases...
22 February 2021 4,374 3 View
I am planning to work on T2 diabetic animals and check the efficacy of probiotics. My initial plan is to fed B6 animals with HFD for a while. But now, I have the chance to work on ob/ob mice. My...
18 February 2021 6,896 3 View
Hello, I am working on a global scale hydrology GIS project delineating global watersheds and doing some data analysis. I have in the past used hydro1K from the USGS that cover the entire world....
18 February 2021 5,109 5 View
I was trying to isolate fibroblastic reticular cells (FRC) and follicular dendritic cells from mice spleen, but kept getting poor yield. I found from literature that the frequency of FDC is around...
17 February 2021 3,506 4 View