As mentioned in the title. Could anybody help to provide some references about these questions?
I assume that you are interested in muscle fiber free cytosolic calcium concentration and not skeletal muscle calcium concentration. If you are interested in the latter, be aware that it varies greatly within skeletal muscle, i.e., from 10-300 nM levels in the muscle fiber cytosol at rest to mM levels in sarcoplasmic reticulum, mitochondria, and extracellular space. Following slaughter, one would expect the free cytosolic calcium level to increase as the level of ATP decreases; this in turn activates a series of calcium-sensitive degradative enzymes. However, I wouldn't expect the overall skeletal muscle calcium concentration to increase following death.
Bing,
Not sure if you have seen some of these references in relation to Ca concn & sensitivity, esp in relation to early PM conditions. some of my favs:
Macintosh, B. (2003). Role of calcium sensitvity modulation in skeletal muscle performance. News in Physiological sciences, 18, 222-225.
Martyn, D., & Gordon, A. (1988). Length and myofilament spacing-dependent changes in calcium sensitivity of skeletal fibres: effects of pH and ionic strength. Journal of Muscle Research & Cell Motility, 9(5), 428-445. doi: 10.1007/bf01774069
Pork specific:
O'Brien, P. J. (1986). Porcine malignant hyperthermia susceptibility: hypersensitive calcium-release mechanism of skeletal muscle sarcoplasmic reticulum. Canadian journal of Veterinary research, 50(3), 318-328.
Should give you a start anyway.
The report about the free calcium concentration in postmortem muscle is not consistent, but it is sure that the free calcium will increase in postmortem muscle due to the function loss of proteins regulating calcium retake. In living animals, the calcium concentration would be around 100 nM for relaxation and at 5 uM for contraction of skeletal muscle. It can inrease to 100-200 um in postmortem muscle.
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