I am going to do in vitro culture of bovine luteal cells. I want to measure these proteins by western blot but I have have protocol belonging these proteins:
1- 3 B hydroxysyeroid dehydrogeanse
2- STAR( steroidogenis acute regulatory protein)
3- smooth muscle actine
Check out this paper for your first target protein:
http://www.ncbi.nlm.nih.gov/pubmed/8477648
The antibody you purchase typically varies from company to company in concentration, precise epitope, etc., so you may have to troubleshoot some. I would recommend BLASTing your target protein to make sure that the antibody's recognition sequence matches your target:
https://blast.ncbi.nlm.nih.gov/Blast.cgi?PROGRAM=blastp&PAGE_TYPE=BlastSearch&LINK_LOC=blasthome
Anti StAR and anti HSD3B from abcam works well for me.
My concern is for an antibody able to discriminate between Cyp11 and Cyp17. and it's not that simple when you know a lot of informations are confidential...
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