How to add a journal?

I would like to know how to register the journal Flora Montiberica (ISSN 1138-5952 - eISSN 1988-799X) in Research Gate.

02 March 2021 8,156 2 View

Is it possible to obtain a negative cronbach alpha?

Good evening everyone. If a questionnaire has 4 factors measuring different constructs as for example CAMI questionnaire measuring attitudes toward the mentally ill that has four factors:...

18 February 2021 3,223 3 View

Antibody amino acid sequence database to simulation?

Hello, I need your help. I am looking for a database of antibody amino acid sequences for later simulation in a program in order to analyze their structure. The antibodies I need to test...

18 February 2021 371 4 View

How long can we consider that the data obtained with qualitative social research methods are "current"?

I cannot find any reference that deals with the temporal validity of qualitative data. I think there is a gap in the methodological literature on the temporal validity of the research results and,...

05 February 2021 9,788 20 View

Does DNA A-tailing survive after agarose gel electrophoresis and purification?

Hi everyone, I need to clone in pGEM-T vector the result of amplifying a metagenomic library by PCR with a high fidelity polymerase but I only want to clone the fragments of an specific size...

04 February 2021 6,051 8 View

How can I solve this problem with agarose gel DNA purification of bigger (1-4kb) fragments ?

Hi everyone, I perform a PCR + Restriction Digestion (single cut, same at both sides) over a metagenomic library. After that, I am trying to purifiy DNA fragments between 1-4kbp long from the...

24 January 2021 2,190 5 View

What is the most adequate (serum) dilution to use to determine IgE in mice serum via ELISA assay (commercial kit)?

We have a commercial kit from RayBioTech to determine IgE from mice serum. In accordance with the supplier's instructions, we have to use a dilution of 1:10000 of the serum. Is this dilution the...

06 January 2021 4,217 3 View

Is there any reference levels of IgE in healthy mice serum, like there are for other biochemical processes/components?

My question goes for reference levels of IgE in mice serum. I know that there are tables for the most common biochemical processes/compounds (red blood cells, white blood cells, AST, ALT, etc),...

06 January 2021 8,215 3 View

Which plants and trees have the best ability to prevent the entry of particulate matter (PM2.5 and PM1) and nanoparticles in indoor environments ?

In recent years our group has highlighted the importance of trees and plants in public and recreation parks in large mega cities. However, we are interested in developing "green shields" to be...

05 January 2021 6,500 3 View

No title

It is important for me to know the opinion from your point of view as researchers.

30 December 2020 988 2 View

What is the significance of (isolated) high titers of IgG anti GD1a antibodies in an older patient with polyneuropathy?

Remote history of Campylobacter infection with reactive arthritis, and Spitz nevus (UMP). Arthropod exposure in Zika/ChikV endemic area prior to emergence of length dependent, sensory PN with...

18 February 2021 8,719 1 View

How to calculate the sample size required to detect the difference in pharmacokinetics of methotrexate?

I came across with this study here, https://www.clinexprheumatol.org/abstract.asp?a=5066 And this study says: "The sample size calculation was done to compare two different MTX starting dose...

14 February 2021 3,017 3 View

How do I prepare and store insect samples for JH measurements?

I need to preserve termite samples for future quantification of juvenile hormone in their bodies. Or extract the Juvenile Hormone and keep that samples stored for future quantification. I may need...

09 February 2021 1,995 1 View

mRNA vaccines against Covid-19 may worsen cronic autoimmune disease?

Example: if a patient suffers of a 15 years psoriatic arthritis and gets Leflunomide (20 mg/ or 40 mg per day) and methilpredisolone (4mg per day). Then, his turn arrives to be vaccinated against...

11 January 2021 7,788 11 View

What about new vaccines for Covid-19 and autoimmune diseases ?

Many people, who are going to be vaccinated soon, suffer a variety of autoimmune diseases (i.e. reumatoid arthritis or psoriatic arthritis and many others). In particular, some of them are...

05 January 2021 6,069 3 View

What is the difference Knee joint Cryo section vs Paraffin section?

I have a tissue that causes arthritis with MIA. Usually everyone uses Cryo or Paraffin section for knee joint. What's the difference?

23 December 2019 8,103 5 View

How effective is warm water therapy in weight loss, diabetes, arthritis, etc.?

Warm water therapy (actually luke warm water mixed with/without lemon juice, salt etc) is becoming very popular among health-maniac netizens especially aiming to weight loss and to cure/manage...

15 September 2019 2,983 17 View

Why cell lines are not prefered for rheumatoid arthritis studies?

29 August 2019 7,973 3 View

Why cell lines are not prefered for rheumatoid arthritis studies?

07 August 2019 1,263 4 View

Are there any biomarker to find the proper treatment for patients affected by psoriatic arthritis?

Cancer research has developed precise biomarkers to use a proper cure for different kind of cancers. Why there is no proper biomarker to find the precise treatment in autoimmune desease, such as...

25 July 2019 9,710 4 View