I'm looking for two older articles on the Hamilton Anxiety Rating Scale (Refs below). Does anyone have?

Hamilton, M. A. X. (1959). The assessment of anxiety states by rating. British journal of medical psychology, 32(1), 50-55. Hamilton, M. (1969). Diagnosis and rating of anxiety. Br J Psychiatry,...

09 February 2020 5,958 3 View

Hello, if the plant extract contains steroids based on its phytochemical results, is steroid an antihyperglycemic agent?

Our plant sample contains steroids based on the phytochemical results, and were having a hard time looking for some references about steroids' effect on lowering blood glucose level on mice.

31 January 2020 9,763 3 View

How to calculate peak fluence for elliptical Gaussian laser beam?

16 October 2019 3,663 4 View

How to mutate a DNA sequence to stop binding to its specific primer?

I am doing reverse transcription using a semi-suppressive PCR therefore I introduce a template switching oligo at the 5' end of my transcript which is complementary to the PCR handle at the 3'...

03 October 2018 4,282 2 View

Need help making a Fiji (ImageJ) Tool

I'm working on manually analyzing a series of photographs of archaeological artifacts. Each photo contains a series of stone tool fragments laid out on a neutral background. I'm using Fiji image...

06 January 2014 5,335 10 View

Any common protocol for inflammatory stimulation of murine endothelial cells in vitro?

26 December 2013 1,701 9 View

Can the measurement of cytokine levels according to their half lives be useful?

A question of cytokine kinetics. In an activation assay, we usually want to measure effects of our treatment (whatever this might be) on pro- or anti-inflammatory cytokines. Most commonly...

28 November 2013 2,096 3 View

MPO activity assay - can anyone help?

I homogenized murine lungs in 0.5% w/v HDAB in 50mM sodium phosphate buffer to perform a MPO activity assay. The protocol I do have instructs that I should use 20ul of my samples with 160ul TMB...

01 October 2013 8,409 3 View

How fast should an ELISA plate be read?

I finished an ELISA assay and read it to discover two hours later that I saved the 630nm data which I used to correct my 450nm data. I immediately measured the plate again but I'm not sure whether...

05 September 2013 4,109 21 View

qPCR data representation

I have some qPCR data which I want to represent in graphs. I used to use the DCt values but the problem is that they are reciprocal to the expression changes (high DCt values means low expression)...

13 July 2013 4,875 2 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View