How to remove water from plant extract??

Hello everyone, I hope these words find you well. I used 80% methanol (solvent) for seaweed's extraction. I have performed rotary evaporator extraction in order to evaporate methanol but there is...

16 October 2020 5,598 8 View

Researchers/ organizations working on gender issues in Vancouver?

Hello everyone, Please reach out if you work on gender studies, gender psychology, social psychology of gender, or gender-based discrimination. You can reach me here or write to me at...

05 August 2020 8,313 7 View

How can we check the real content of calcium in the protein samples?

03 August 2020 4,309 3 View

Capability of MCNP6 in Muon tracking?

Is MCNP6 able to simulate galactic cosmic ray specifically Muons ? if yes does it have any energy or cross section restrictions?

16 June 2020 9,049 3 View

How can I transform dichotomous data into continuous/ Likert?

Hi RG family members, Can someone advise me on how to transform my dichotomous data into continuous variable in SPSS? Best wishes, Gulnaz

08 April 2020 9,849 9 View

Can anyone provide or suggest some reliable articles on second order SEM modelling using SmartPLS.?

Hi everyone. I need some article to understand the second-order SEM modelling using PLS. If anyone can suggest or can share I shall be highly grateful.

03 March 2020 8,605 6 View

Help me construct a research topic on : open drug market and rising spate of drug abuse?

i need assistance on to construct a better research topic on the role of drug market on rising spate of drug abuse.

28 February 2020 8,251 3 View

Can I use an E-Beam patterned Photoresist as a mask for UV photoresist exposure?

Dear Seniors, I am wondering, if I could expose my pattern in E-beam resist (Suggest me one for this purpose) which is on top of UV photoresist (AZ some suitable one). Then I used this written...

21 February 2020 9,650 1 View

Assessment of air microflora in the microbiology Laboratory?

To assess the air microflora in indoor air of laboratories

14 January 2020 2,006 4 View

Which international universities hold the Digital Transformation DBA course?

Hi dears, I want to learn more about Digital Transformation and its impact on Business model, strategy, marketing, agility and ... .I want to apply for online course at universities; is there any...

24 December 2019 3,817 11 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

How to best wash a 96-well plate containing microplastic?

I am planning my protocol for my dissertation for an experiment quantifying bacterial (E. coli) biofilm formation on micro-plastic (5mm discs of polyethylene bags) by conducting a crystal violet...

23 February 2021 7,868 2 View

How can I improve the concentration of banana fruits extracted RNA?

Currently, I'm working with banana fruits. I've tried to do RNA extractions with different methods, and I've gotten total RNA without contamination, but I couldn't get a high concentration of...

22 February 2021 6,126 2 View

Color of spiro-ometad film?

What does the color of a spiro-ometad film indicate? I got purple, green, red, even black color of the spiro film. Is there anything we can get from the preliminary color analysis(e.g. oxidation...

21 February 2021 1,831 4 View

Has anyone encountered any problems with abm good microRNA primers for quantification of mature miRNAs?

I've been trying to quantitate mature microRNAs in 293T cells with and without 'X'. 2ug of total RNA (isolated by Trizol method) is converted to cDNA using All-in one microRNA cDNA synthesis kit...

17 February 2021 8,986 2 View