Is anybody using PetroOXY to analyze lipid oxidation? How do you compare with rancimat interms oxidation mechanism?

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How to solve the problem of unconscious teeth grinding during sleep at night?

One of my friends, every night after falling asleep, his mouth would make a creaking sound after chewing something. This teeth grinding condition would continue until he woke up, and he didn't...

08 August 2024 1,366 5 View

Is Galaxy.org good to use for research for analyzing data and for publication?

Hello all, I wanted to know, can I use galaxy (USA, Europe or Australia) platform for analyzing the shotgun data, and can it be used for publication purpose as well? Thanks :)

06 August 2024 6,610 4 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to send my account link and how to make my account public?

Nothing

01 August 2024 755 1 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

Should Coefficient of Variation (CV) in a biological data necessarily be below 30%?

In a research, lesion diameter due to a disease has a CV below 10%. However, in an another parameter, disease inhibition percentage (compared to control), a value derived from lesion diameter...

27 July 2024 201 0 View

Non-parametric version of the wo-way repeated measures ANOVA?

Hi, can someone please help me with their expertise. To analyse a dataset with 2 IV´s (2 levels each) and 1 DV (time - measured at 4 points, i. e. 4 DV?) I found the two-way repeated measures...

24 July 2024 3,284 6 View

Is possible to edit a gene present in a plasmid by Crispr Cas9?

Hello everyone! Someone working with Crispr Cas9. I have a question. Is it possible to edit a gene that is present on a plasmid (in this case with a low copy number)? In this case, is it possible...

22 July 2024 7,073 2 View

How to determine tannin content in fodder pellets?

22 July 2024 8,163 3 View

To what extent are artificial intelligence techniques used to enhance science learning in schools in the Arab Gulf countries???

I want researchers who specialize in studying artificial intelligence in the field of science education and those related to it to give me information about the application of the...

18 July 2024 8,320 0 View

Could dyes amplify the spectrum of light to a specific wavelength?

I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...

05 August 2024 3,290 1 View

Optimal siRNA Volume for Complexation with Liposomes in a 2.5 mL Solution with 10 mg/mL Lipid Concentration?

How much volume of siRNA can I add to 2.5 mL of liposomes with a total lipid concentration of 10 mg/mL?

27 July 2024 6,920 1 View

Can we detect DOC fractions through LC-OCD for a sample containing Sodium Azide?

I did some column tests in my lab where I needed to detect the DOC fractions using LC-OCD. For the abiotic columns I used sodium azide to restrict the biofilm formation. I did LC-OCD to detect the...

07 July 2024 468 0 View

How to perform the DFT calculation on the mechanism of Photocatalytic CO2 reduction with water?

Dear Researchers, I hope this email finds you well. I am currently working on research involving DFT calculations to study the mechanism of CO2 reduction using a catalyst with water as the...

14 June 2024 4,612 4 View

Curing of Novolak resin with hexamine develops yellow color. I believe it is due to azo linkage -C-N=N- C- What is the reaction mechanism?

Also, I will appreciate whole curing mechanism of Novolak with Hexamine

14 June 2024 9,908 0 View

How to stain lipid droplets using bodipy?

I opted bodipy due to its smaller stoke’s shift (493/503) but my images do not have consistent results. in the same well few fields are clean without background and other fields have background....

11 June 2024 3,463 3 View

What is the Standard Procedure for measuring Total Lipid concentration in Daphnia magna?

currently working on a project involving Daphnia magna and I am intertied in measuring their total lipid concertation. I was hoping to get some insights or advice on the standard procurers for...

23 May 2024 8,846 1 View

Making glass slide/coverslip hydrophobic?

Hi I have been working on droplets, lipid vesicles for my experiment and I have a serious issue that my droplets get stuck on the coverslip. I use the following protocol below to make my...

21 May 2024 9,285 3 View

Advanced Oxidation process ?

Advanced oxidation process is usually produces carbon dioxide , is it harmful to run such setup in the lab?

17 May 2024 6,550 3 View

How can I combine MXene (titanium carbide) with rGO so it will provide high Absorption rate (formula: A=1-R-T , the value of A must be ~ 0.9)?

My task is to achieve maximum absorption and shielding effectiveness. I mixed Mxene dispersion with rGO dispersion in different ratios and tested them with VNA. I chose rGO as a good absorber and...

06 May 2024 2,643 3 View