I am going to investigate the growth of Celeribacter spp. ( isolated from marine environment) at different salt concentration by using Luria Bertani broth. I was wondering if anybody could tell me, what other ingredients except trypton, yeast extract, NaCl should be added to LB?
This website might be helpful:
http://www.microbelibrary.org/component/resource/laboratory-test/3031-luria-broth-lb-and-luria-agar-la-media-and-their-uses-protocol
It notes that Vibrio species have been isolated using LB with 30 g/L NaCl rather than the more typical 5 g/L . The site also has the standard recipe for LB and some variations.
We often use difco marine broth for cultivation of marine bacteria. You can find the formula here:
http://www.protocol-online.org/biology-forums-2/posts/32416.html
I think you should stick as close as possible to the salt composition of ocean water and vary only NaCl concentration for investigation of the response to osmotic stress.
How much salt have been used? You can also added caCl2 and glucose to medium from 1-2 g in 1l to enhance the growth.
Thank you for your answer. I am going to investigate different NaCl concentrations of 0.5, 1, 2, 3, 4, 5, 6, 7, 8, 9,10, 12, 15, 18 or 20 % (w/v) by using LB.
You can add 1 or 2 % of glucose along with yeast extract and add some inorganic salts (NH4)2SO4, KH2PO4 , MgSO4∙7H2O , CuSO4∙5H2O , FeSO4∙7H2O, MnCl2∙4H2O , ZnSO4∙7H2O into the medium with different percentage of Salt and observe the result.
I suggest for marine bacteria isolation or culture to use more suitable medium than LB and you can use Marine Broth of Difco or add marine filtered water to prepare medium. Else, I agree with Jurgen and Karthick to try these formula
I am also used LB for my study its not working well, i got better result in Marine broth and minimal media composition which i gave in the previous comment. My suggestion is better you try with marine broth its suitable for culturing marine bacteria........
would youplease tell me exact amount of yout formula karthick for LB
Ready made LB broth contains Casein enzymic hydrolysate 10gms, Yeast extract 5gms and NaCl 10gms/lit and I added 1 to 3% glucose and NaCl at different concentration 1 to 10 for my work it doesn't work well compared to other medium. Marine broth contains yeast extract 1gms and 19.45 gms of Nacl/lit its sufficient for the bacterial growth but for your work you try accordingly to your strain because if you add more NaCl it restrict the growth of some bacteria. So try how much Salt is needed for your strain like 22gms in liter is sufficient means you add 1-5% more Nacl, beyond that it will not work.
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