Does anyone know of reliable antibodies for phosphorylated TRPV1?

02 April 2020 0 7K Report

I am interested in performing western blot on mouse tissue and am looking for commercially available antibodies for phosphorylated TRPV1.

Badges
Science topic

Similar topics
Eutheria
Rodentia
Muridae
Murinae
Mice

More Jami Saloman's questions See All

SPRKKR for interstitially doped alloy, .sys created by cif2cell?

Dear SPRKKR users, I was trying scf SPRKKR calculations for an interstitially doped Heusler alloy and I am getting the following error in the output file : VAR(2) = 0 for (T,N,L,K,M;S,NSOL)...

04 September 2023 6,765 0 View

How to interpret principal stress calculate from rosette strain gauge?

Hi, I used a rosette strain gauge to measure the strain on a beam. From these measurements, I'm trying to calculate the principal stress values, but I'm not sure if the result makes sense. I...

17 July 2022 8,965 0 View

What are the ways to measure Curie Temperature using ab-initio techniques(in VASP) or Green's Function based method?

Curie Temperature using ab initio technique; basically computational way

09 July 2021 7,596 6 View

How can I get dataset of 5G cellular network with parameters like user distance from BS, Time taken, etc?

I need a dataset for classifying users based on distance and time taken ( with delay spread). So I'll be happy if I get some suggestions or existing datasets. Thanks in advance.

24 March 2020 9,145 8 View

Simulation of hammer impact test to obtain free vibration response on a cantilever ?

Hi Is there any software that I can simulate the impact test (with predefined force) on a cantilever and measure the free vibration response or FRF signal? I need acceleration /or displacement...

23 May 2019 7,787 3 View

Who are some of the good English-Urdu and Urdu-English translators in the literary history of Pakistan?

Translation in Pakistan.

11 December 2017 3,949 0 View

Any suggestions on the inconsistency between blast and phylogenetic tree?

I did nearly full 16S sequencing (1382 bps) of my isolate. According to blast result in EZ taxon data base the isolate showed 98.5% similarity to Kocuria polaris but when I drew phylogenetic tree...

17 March 2015 3,393 12 View

Do you think TSA could be a suitable media for purification of presumptive actinobacteria?

I isolated presumptive Actinobacteria from 3 media (Kuster agar, SCA, AIA) then put them in BHI broth at -80 . I am going to purify them on TSA.Do you think that could be a suitable media for all...

24 February 2014 8,430 6 View

Anyone know of what affect Cysteine-HCl has on culture media?

I added Cysteine-HCl to MRS and Colombia Blood Agar to reduce O2 of the media. could any body tell me should I store the media under anaerobic chamber because of Cysteine-HCl or it does not make...

30 January 2014 4,271 10 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Does anyone know what might be causing the smeared bands on my western blot?

I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...

10 August 2024 7,480 3 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Microtome tissue sectioning - How to prevent chatter of paraffin tissue specimen sections?

Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...

06 August 2024 666 4 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View