See above.
I have heard that some lab strains lost phage sequence. sorry, cannot say more...
For elemental phase (= terminal solid solution based on one metal element) consisting of one major element, Yeh et al. [1], Murty et al. [2] and Gao et al. [3] proposed a small-negative enthalpy...
21 January 2023 522 4 View
Hi, I did some 1H NMR measurements to analyse bacteria-infected urine (yellow graph). I have a clear peak at 1.93 ppm (according to literature: acetic acid). However, I cannot identify the peak...
31 March 2022 2,179 1 View
Is there any publication that has investigated how long it takes for nectar to dry within a cell inside the hive, before it is capped? I am aware that it depends on multiple factors such as the...
31 January 2022 439 2 View
Hello everyone, I'm trying to mesh a structure with complex partitions using mesh controls. When doing it on the user interface I'm first selecting all cells. In the following, I have to accept...
03 February 2021 8,600 1 View
Hey there, I am currently working on the statistics of counted and expected genotypes in mice litter of a mutant mouse model. I have two different mutated genes, both alone and in combination, one...
03 February 2021 6,753 2 View
Our transgenic mice show a really strong phenotype which differs a lot from results of another group working with the same transgene. As our mice are on a pure C57Bl6J background and theirs was on...
24 September 2020 2,060 3 View
I was wondering if anybody could give some tips for B cell culture (primary splenic B cells, MACS purified, 5x10^5/ml + 8ug/ml LPS, + 20ng/ml IL-4, in RPMI, 1-2 days). It seems that I can use...
13 July 2020 6,596 0 View
Dear all, I have the following problem: I want to run a co-citation analysis of a certain set of publications. However, some of them are not part of the Web of Science database. Is there a...
13 December 2019 9,839 7 View
I am trying to transduce primary murine cerebellar granule cells using a lentiviral system. I tried transduction with polybrene, but it seems to be quite toxic for my cells. However, transduction...
10 November 2019 8,691 6 View
Hello you all, we want to perform an Annexin V and PI staining on our B cell culture, does anybody know if using the commercial buffer (biolegend in our case) is absolutely necessary? Or can we...
14 May 2019 2,733 3 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
Hi, I wanna to implement evolutionary algorithms in ryu sdn controller in mininet, i have some challenges, how i can run the big scale topo with one sdn contoller??? and another question is to...
21 July 2024 246 2 View
is there any ways that i can find genome similarity of an organism without whole genome sequencing ,like using maths formulas or experimental progress ?
05 July 2024 4,070 3 View
In the host range analysis, I saw that a temperate phage that I isolated had an effect against a different strain, but later (2 years) it did not affect that strain. What could be the explanation...
25 June 2024 9,729 3 View
After incubation of the TG1 cells with the helper phage, I spun down the culture and precipitated the supernatant with PEG/NaCl. After this, I spun it down again and resupended the pellet in PBS....
21 June 2024 6,260 2 View
It is known that patients with desminopathy often die from pneumonia. Have pathomorphological studies of the lungs been performed in patients with desminopathy?
15 June 2024 1,355 7 View
I found a variant segregating with a genetic condition, this variant is listed in ClinVar (with no clinical significance), gnomAD, etc. However, I could not find any publication reporting the...
10 June 2024 7,606 3 View
Hi, I am trying to make synthetic phage library. Right now , do people still use Kunkel method or other methods? Since it looks like Kunkel method is an old method. I am looking for more...
09 June 2024 6,780 1 View
Describe the principles of Population Genetics and evolution as applied to livestock breeding programs
08 June 2024 2,924 0 View
Recently, The isolation of bacteriophages from sewage water is so difficult, How to obtain phages plaques when i make isolation bacteriophage experiments for these bacterial types as bellow; 1-...
07 June 2024 9,473 1 View