I am using RNAscope Multiplex Fluorescence assay for RNA ISH, the protocol for fresh frozen tissue requests using a RNAscope probe dilution product to dilute the probe of interest from a 50x to a 1x working solution. A colleague of mine mentioned they use an off target probe to dilute their 50x. Thoughts on whether the negative control probe (a bacillus subtilis gene targeting probe) could be used as a probe diluent for a DRD1 mouse brain probe?
(trying to spend less while I optimize the RNAscope protocol for my tissue)
Hi Nicole,
I have the same problem, I will need to run the assay with a C2 probe only in two days and i don't have the diluent. Did you find a solution?
thank you!
Lucia
Hi Nicole, Hi Lucia,
I find myself in the exact same situation, did you eventually try using the negative control probe to dilute a 50x probe?
Thank you!
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