PLS-SEM generalizability issue?

I'm developing a readiness assessment model regarding contractors' preparedness for a specific activity, in order to do so, a survey study was carried out and the data analyzed with PLS-SEM to...

24 November 2020 399 1 View

What are these bioclasts?

I'm having trouble identifying the round bioclasts indicated by white arrows. Could they be transverse sections of the elements indicated by black arrows? If so, could they be some kind of spicule?

22 July 2020 8,595 13 View

Is possible to run models with catergorical response variable and random factors?

Dear all, I would like to know if is possible to run a model with a categorical response variable plus some categorical predictors (and its interactions) and a random factor in R? If yes, any...

26 March 2020 1,221 3 View

Are effect size estimators Spearman's rho and Pearson's r interpreted in the same way?

Hello! Can classifications of effect sizes for Pearson's r can be used equally for Spearman's rank correlation rho? For example, in Ellis (2010): r = .10 -> small r = .30 -> medium r = .50 ->...

25 February 2020 1,046 3 View

Could you help me in defining the time-lag in a longitudinal study for measuring the impact of a purpose program?

23 February 2020 7,744 3 View

How to deal numerically with the discontinuities coming from a Butler-Volmer interface condition in a secondarry current distribution model??

Let us suppose that we want to compute the secondary current distribution of an electrolytic cell composed by anode, electrolyte and cathode. The governing equation in each subdomain isgiven by...

17 February 2020 7,913 3 View

Low RNA yield after targeted RNAse H?

Hello folks, I am trying to cut a specific RNA using RNAse H. To do that I'm annealing an oligo which targets my RNA (annealing buffer TRIS 10mM, NaCl 50mM, 1mM EDTA) heating the mix (with...

03 February 2020 6,689 2 View

How to install YAML.pm?

I'm trying to annotate a yeast's genome using FunGAP but it stopped in the run4 of Maker. The gmes log said that I may need to install YAML module, as follows: Can't locate YAML.pm in @INC (you...

26 November 2019 7,121 2 View

How can I create a new dataset from the subtract of two datasets in EEGLAB?

17 June 2019 4,532 3 View

What is the maximum specific surface area for an inorganic material?

If you take an inorganic material like a mixed oxide cerium-zirconium. (1) May we say that there is a natural limit in the SSA (m2/g), e.g. 200 m2/g? My understanding is that there is a...

28 March 2019 4,836 4 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Experimental Design: How deal with pseudreplicates with immunological and clinical-chemical paramaeters?

Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...

24 February 2021 1,243 1 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Does PHA-P stimulate the proliferation of lymphocytes from mice?

Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...

23 February 2021 5,476 4 View