I want to make aliquots of 8hydroxy2'deoxyguanosine and want to freeze them for future use. Which storage temperature will be good for stability? -20 or -80oC?
since your freezing DNA matter i'd be concerned what the effects of thawing have. -20 or -80 what are the effects?
I am new to research. The boiling point of triethyl amine (NEt3) is 89 degrees centigrade. I am skeptical that when I will let the hydrogen escape which is getting generated in situ, I will also...
21 July 2024 7,284 4 View
What is the best 'free of charge' software for extracting GPS data from a video created from a GoPro camera? I have checked the Telemetry Extractor...
02 June 2024 3,138 2 View
How to modify a metaheuristic algorithm to obtain a better-performing algorithm?
28 April 2024 3,627 4 View
effects of elovera on the efficiency of transplanted endothelial cells
28 December 2023 1,994 0 View
We are trying to revive an old Raman Spectrometer from Lambda Scientific. This is a LIRA-300. I was wondering if someone had the drivers and the software of this now obsolete system. In...
04 November 2023 5,129 0 View
How do I run a mediation with two independent variables and one dependent variable in PLS SEM? Please suggest some paper as well its interpretation ?
28 October 2023 3,857 1 View
Immunohistochemistry of MMP-2 and MMP-9 expression in dibetic wound healimg
24 October 2023 2,449 1 View
Elder population, infants, and pregnant women are at the risk of ICU admission leading to deàth. In such cases, it is neccessary to recognise the role of accidental hyperglycemia linked with...
03 January 2023 5,982 2 View
Greeting Respected RG Scientist and Researcher I submitted a minor revision for my paper based on the comments by the editor and Reviewers 1 and 2. in Hindawi publisher. It has been over two...
31 December 2022 1,626 15 View
hello everyone I need an inexpensive methodology for the production of biochar from sugarcane bagasse by adopting slow pyrolysis techniques with temperatures 200 and 300 degrees C with residence...
11 November 2022 119 5 View
I am puzzled about the properties of gelatin, when used for tissue-embedding. Hard gelatin can be melt again by temperatures about 40°C. Formalin-fixed gelatin is like crosslinked protein and...
07 August 2024 1,686 1 View
Do these peaks in the curve indicate primer dimer or non-specific products?
01 August 2024 7,466 0 View
What is the best way to freeze the rat brain - liquid nitrogen, Dry ice , isopentane - after isolation for later protein investigation by Elisa?
01 August 2024 1,722 1 View
Samuel Morse, the inventor of the Morse Code, understood that certain letters in the English language occurred more frequently than others (Gallistel and King 2010). To deal with this, Morse used...
01 August 2024 4,456 2 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View
90% of museum objects worldwide are in storage.
21 July 2024 8,038 6 View
Hello everyone, I am currently working on a project involving spatial transcriptomics on E14 mouse embryos. We need to perform cryosectioning without fixing the embryos. Here is the protocol we...
10 July 2024 5,985 0 View
My study uses C57BL/6J mice and I am required to store portions of the important organs in neutral buffered formalin (NBF). Could I potentially keep those tissues in the formalin for a few weeks...
06 July 2024 8,360 2 View
What is meant by cross-contamination and what causes fruits and vegetables to become contaminated during preparation and storage?
02 July 2024 9,204 0 View
In a post-harvest handling chain (harvesting, transportation, storage), which among these do you think has the highest percentage loss why?
02 July 2024 7,649 0 View