We are measuring mice blood glucose level using glucose meter for human, we sample the blood via tail nicking, sometimes the yellowish, translucent fluid comes out (Interstitial fluid) and its reading on the glucometer is much higher than that of the whole blood. This makes me wondering if it mix with the whole blood from the tail nicking, will it substantially affect the supposed blood glucose level reading on the meter?
Hi
Self-monitoring of blood glucose was described as one of the most important advancements in diabetes management since the invention of insulin in 1920. Recent advances in glucose sensor technology for measuring interstitial glucose concentrations have challenged the dominance of glucose meters in diabetes management, while raising questions about the relationships between interstitial and blood glucose levels. This article will review the differences between interstitial and blood glucose and some of the challenges in measuring interstitial glucose levels accurately.
Regards
Article A Tale of Two Compartments: Interstitial Versus Blood Glucos...
Interstitial Fluid
The fluid that surrounds the cells of multicellular animals. The advent of sensors that can measure glucose in the interstitial fluid has allowed companies to develop devices for continuous glucose monitoring. However, because interstitial glucose levels are not identical to blood glucose levels, continuous monitoring devices (CGMs) must be calibrated with a blood glucose reading from time to time. Additionally, people using a continuous device may find it helpful to occasionally check their blood glucose with a conventional meter to confirm interstitial measurements.
A number of glucose monitoring systems are now on the market, including those manufactured by Abbott, DexCom, and Medtronic. Each of these systems consists of a glucose sensor placed under the skin and replaced every 3–7 days and a receiver that stores interstitial glucose measurements. Continuous glucose monitoring can detect trends in glucose levels as well as alert the user of high and low glucose levels. These trends and readings are displayed on a screen on the receiver.
http://www.diabetesselfmanagement.com/diabetes-resources/definitions/interstitial-fluid/
Jiyao, how certain are you about the nature of this yellowish translucent fluid? It seems to me that you may have a damaged or infected tail around the area where you previously snipped it.
Try a 30 G needle to prick the tail at location that is quite distal to the damage.
Portable glucometers are calibrated for blood and normal hematocrit - without fulfilling these requirements, you cannot get reliable numbers on blood glucose.
- Badges
- Science topic
- Similar topics
- Computer Science and Engineering
- Bioinformatics
- Bioinformatic Tools