Good evening, can you help me interpret the results of the 1% agarose gel.
DNA was extracted from plant tissue using the CTAB protocol. In the wells (left to right) in well 1 and 4 is the molecular weight marker (1kb) with the amount of 1μl, well 2 has 1μl of sample + 1μl of loading buffer. well 3 μl of sample + 1μl of loading buffer.
Electrophoresis conditions: 80V x 30 min.
I am mainly interested in understanding the banding pattern of well 2.
Thank you.
It looks like you have undigested RNA in 2 well. Have you added RNAse?
Hello Andrea, the first band in the well 2 and 3 indicate the presence of DNA. Large and faint smear in front of these band may be RNA or other impurities.
2. ladders are often use to know the size of gene (usually PCR product). In this case, I don't thing 1kb ladder is useful when we detecting presence of genomic DNA.
Most folks don't bother to run out gDNA on an agarose gel since it's not informative unless you are doing a very specific protocol like long-read DNA sequencing where you need to verify intact, large DNA molecules. If needed, measure the concentration in a spectrophotometer (aka nano drop) or Qbit.
Typically, you just proceed to the next step in the protocol.
Good luck!
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