Interpretation of Microscopic picture of MCF cell lines?

More Vivek Junghare's questions See All

Can anyone identify the tick species ?

Can anyone tell me the tick species

13 July 2024 7,146 6 View

Journal's Impact Factor: Publication or Updated?

There are two scenarios: The Impact Factor at the time an article is published. The Impact Factor after the journal updates its metrics annually based on performance. While being interviewed or...

25 June 2024 7,824 2 View

Can anyone explain how mobile ions moves inside ploymer electrolytes?

I want to know the exact mechanism behind the motion of ions inside the polymer electrolytes.

13 June 2024 3,489 0 View

Funding sources in India to attend international conferences (in abroad)?

Being a Ph.D. Scholar, I am seeking funding sources that offer financial assistance for conference registration and travel expenses to attend international conferences, specifically those held...

26 May 2024 711 2 View

I'm facing serious problem about sample size determination please help me?

I'm doing research in veterinary science and topic is associated with ticks prevalence and seasonal variation in cow..I have randomly selected 560 cows as sample size and studing for prevalence...

25 May 2024 3,834 5 View

SHA-256 Hash function transmission?

I have applied the SHA-256 hash function to the plain image to generate the initial values of the chaotic map in the image encryption algorithm and include it in key space analysis. So, when the...

10 March 2024 8,854 4 View

I am getting higher seismic demand in terms of displacement for FRP confined RC column as compared to RC column?

I am using concrete01 and the stress-strain parameter is calculated using Lam and Teng model for FRP confined concrete. How to correct the model so that demand for FRP retrofitted will become less.

05 March 2024 7,328 0 View

Rapidly publishing SCI journals with low impact factor?

Area: Manufacturing, additive manufacturing, CNN, mechanical engineering

09 February 2024 7,943 3 View

How to export RGA data in Partial Pressure (PP) unit in FabGuard explorer which is used for INFICON XPR3 RGA?

I am using Inficon Transpector XPR3 and fabguard explorer software to analyze gases in our vacuum system. I am facing an issue in exporting the RUN data in other units. I can see that I can switch...

24 January 2024 5,303 1 View

Benefits of Wohler's report 2023?

Has anyone gone through the Wohler's report_2023 yet? Its pros and cons? What are the ways to obtain its e-copy? Its subscription is very costly for a normal researcher (around 750 USD per user)....

15 January 2024 8,303 2 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,114 1 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,822 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Is there anyone with experience in TEM analysis who can assist with a manuscript for an upcoming journal?

Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...

07 August 2024 9,561 0 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,105 4 View

Why activated CAR-Jurkat cell could not kill targets?

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06 August 2024 639 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

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04 August 2024 1,200 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

David G Spiller

There is not enough information in your question to be sure but I suspect that you have one live cell in the picture (at the bottom) all the other cells are dead.

Prashant Sharma

Apoptotic and necrotic cells.

I agree with David G Spiller, You should give more information about the question.