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More Brady D. Lund's questions See All
Why wont my PCR product show up on my gel but when sent for sequencing it was successfully sequenced?

After DNA isolation and PCR of my endophytic fungal isolate, neither the gDNA nor the PCR product showed up on the gel but when tested on the nanodrop both displayed high concentrations of DNA. I...

20 June 2023 579 2 View

Post hoc analyses for mixed models: individual models or emmeans contrast analysis?

When conducting post hoc tests for mixed models (lme4 package), the most commonly cited method is to use the package "emmeans" which conducts a contrast analysis. I have slowly shifted towards...

20 December 2022 919 3 View

Why does a precipitate form when taxol is added to tubulin?

I'm running a teaching lab where students are adding taxol dissolved in DMSO to a reaction of tubulin, ddH2O, DMSO, and a buffer (PIPES, 1M MgSO4, water, 0.5 M EGTA, pH adjusted to 6.9, and GTP...

25 October 2021 9,214 1 View

How would I interpret the residuals from a post hoc test on chi-square results?

Following a previous question I posted about statistical analysis for a table (image included), I conducted a chi-square test - χ2 (6, N = 317) = 28.78, p < .0001. As these results were for a...

24 March 2021 7,021 5 View

What, if any, statistical analysis would be best for the summarised data in the included image?

My current study explores the psychology of how children learn about other nations through the media and is examining a television series with anthropomorphised animals. The first stage involved...

23 March 2021 7,919 2 View

Does anyone know of a library for R that includes phi?

I'm looking to produce some phi correlations and I'd rather not do it manually, but I can't find a library for R that includes phi.

18 January 2021 3,255 9 View

Does anyone know how to add custom text to a violin plot in r, ggpubr made using the ggviolin line?

I've produced a violin plot for a point-biserial correlation. Main title is there, x and y are labelled, but I want to add a custom text box with the correlation coefficient and significance...

26 December 2020 4,913 4 View

How do I interpret these correlations and p-values?

I've just run a series of point biserial correlation tests in R between whether or not characters were assigned national identities, and attributions given to their behaviours - results shown in...

17 December 2020 10,226 4 View

When examining a series with different numbers of episodes per series, should I treat it as single sample, use harmonic mean or some other approach?

I'm currently examining how the behaviours of fictional characters are explained to child audiences through attribution theory as part of a study into the psychology of how children learn about...

27 November 2020 8,602 3 View

Any advice for reducing autofluorescence in spleen tissue?

Hello, I'm having some trouble with visualizing my IF in spleen tissue. I first found extremely high background and could not differentiate fluorescent cells and background. I then realized my...

23 July 2020 2,260 1 View

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"A Markov-like Model for Patient Progression"?

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