Any recommendations for Which Insulin to use for Glucose uptake in 3T3 adipocytes?
The one we use is only potent at VERY high concentrations.
We use this one in culture:
http://www.sigmaaldrich.com/catalog/product/sigma/i9278?lang=en®ion=US
You can also use the same insulin that humans use (i.e. Humulin R-100) which is usually around 700 uM stock. We actually have compared both at multiple concentrations in a number of assays, and they give exactly the same results.
Humulin or other clinical human preparations do not report exact concentrations and have other proprietary additives that may skew your data. These should be avoided for use in cell culture at all costs. Order cell culture grade porcine, bovine or human insulin from sigma all would be appropriate for 3T3L1 experiments.
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