Lael Werner

3 Questions 10 Answers 0 Followers

Questions related from Lael Werner

Insulin to use for Glucose uptake in 3T3?

Any recommendations for Which Insulin to use for Glucose uptake in 3T3 adipocytes? The one we use is only potent at VERY high concentrations.

11 February 2016 8,675 2 View

Does anyone have suggestions for the identification of an unknown protein?

I would appreciate suggestions on how to identify an unknown protein. Let's say I have serums from 2 animals (either with or without treatment). When I place these serums on cell-lines which are...

08 October 2015 5,421 3 View

When we used 2-NBDG for Glucose uptake in 3T3 cells, we got a lot of background fluorescence, why could this be?

When we used 2-NBDG for Glucose uptake in 3T3 cells, we got alot of background fluorescence (Perhaps due to binding to the membrane outside??) This made quantifying uptake using a fluoremeter...

01 October 2015 9,224 6 View