When we used 2-NBDG for Glucose uptake in 3T3 cells, we got a lot of background fluorescence, why could this be?

When we used 2-NBDG for Glucose uptake in 3T3 cells, we got alot of background fluorescence (Perhaps due to binding to the membrane outside??) This made quantifying uptake using a fluoremeter...

01 October 2015 9,173 6 View