Hello all,

Rabitt's antibodies against antigens of a sort of Aspergillus species are intended to assess the performance of double diffusion agar.

I would like to know how to develop a protocol intended to immunize rabbits with Aspergillus antigens: how to get a non-letal inoculum from a culture of aspergillus? how often inoculate the rabbit? how much volume? how to create a pool of antigens, for instance 5 types of aspergillus to inoculate? etc...

I hope you can resolve my questions or give more information

thanks in advance!