Hi all.
I am planning to give mouse an I.P injection of insulin at a dose of 0.75 IU/kg, but if a mouse weighs, for example, 20 grams, the dose would be 0.015 IU per mouse. How can you possibly measure 0.015 IU using a commercial insulin syringe?
Hey Yinan,
This is what I do.
I prepare Insulin at 0.1 U/ml in advance.
Injection volume into peritoneal of mouse = BW(g)X7.5 ul of 0.1U/ml insulin solution.
Good luck
Hi Dina
Thanks for your reply.
How did you get 7.5 ul? Isn't it 0.75 ul?
This is to get 0.75 U/kg
you dont, you dilute the insulin so you can take reasonable volume with the amount you plan to give
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