29 June 2022 0 9K Report

Hello

I want to design an aptamer for one of the proteins. If someone has experience in designing using SELEX or any other tool is highly encouraged to share information in details.

More Waheed Iqbal's questions See All
Do you think can be any Uranium bearing rocks in Eastern part of Iran and western part of Afghanistan?

I want to know more about Uranium ore deposits in world.

11 August 2024 6,720 0 View

Do you think can be any diamond bearing rocks in Eastern part of Iran and western part of Afghanistan?

I want to know more about diamond ore deposits in world.

11 August 2024 2,167 1 View

What is the difference between mathematical R^4 space and physical 4D unit space?

We assume that the difference is huge and that it is not possible to compare the two spaces. The R^4 mathematical space considers time as an external controller and the space itself is immobile in...

10 August 2024 6,678 14 View

If Banks do not provide credit facility, what are the options available for FPOs and impact on producer’s income?

If Banks do not provide credit facility, what are the options available for FPOs and impact on producer’s income?

10 August 2024 8,198 5 View

Controlling for pupil light reflex when analyzing pupil size time course?

I used eye tracking to examine how participants from two different populations (A and B) react to an image. Participants in population A exhibit larger pupil sizes over time, but they also have...

10 August 2024 3,229 0 View

What are a “Farmers Producer Organization” (FPO) and its essential features?

What are a “Farmers Producer Organization” (FPO) and its essential features?

10 August 2024 477 5 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

Do interactions between biosphere, carbon cycle, & water cycle impact global warming & interaction between atmosphere & hydrosphere?

How do interactions between the biosphere, the carbon cycle, and the water cycle impact global warming and interaction between the atmosphere and the hydrosphere?

09 August 2024 3,291 2 View

How to get moment output in Abaqus Standart?

I have input a moment load in module load Abaqus, i put my moment load on the node surface (using reference point). I have define moment in history output and make a set for moment too. But the...

08 August 2024 4,831 4 View

How is energy cycled through the Earth's climate system and how do matter cycle and energy flow through the rock cycle?

How is energy cycled through the Earth's climate system and how do matter cycle and energy flow through the rock cycle?

08 August 2024 8,162 0 View

Similar questions and discussions
Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

Seeking Software Recommendations for SELEX NGS Data Analysis?

I am looking for software to help analyze SELEX NGS data, including alignment, sequence enrichment, and other related tasks. Can anyone recommend suitable tools or software? Best wishes, Waleed

30 July 2024 1,061 5 View

How to prediction 2D to 3D aptamer?

Hello, I have a little problem. Well, right now I only have the DNA aptamer sequence. I went to see the 2D model form with DNA folding. After that, I used RNA Composer to simulate in 3D and used...

30 July 2024 6,794 0 View

How to avoid background signal in screen printed electrode?

Hi, I am doing aptamer based electrochemical biosensor. First, I cleaned screen printed gold electrode using 0.5M H2SO4. Then, immobilized aptamer followed by blocking using 2mM MCH. I use...

06 July 2024 9,852 1 View

The failure for thiolated -Aptamer binding onto Gold surface?

Hello. I have try to immobilize the thoilated aptamer onto the gold surface such as Gold coated silicon wafer and the gold coated screen printed electrode. However, it is failed, after aptamer...

29 April 2024 4,054 1 View

Aptamer purification after PCR amplification?

What is the best way to obtain high-performance aptamers after performing sequence amplification by asymmetric PCR other than biotin and streptavidin?

17 April 2024 6,973 2 View

Problem when use autodock tool?

I used the autodock4 tool for docking the aptamer and the ligand. I prepared the DNA aptamer following the process: sequence -> predict the 2d structure by MFold -> predict the 3D structure...

31 March 2024 2,772 0 View

What tool should i use for aptamer - small ligand docking?

molecular docking, aptamer

27 March 2024 4,622 2 View

What best protocol can be used for covalent immobilization of aptamer on polymer membrane surface?

I have confusion in required Temperature.

25 March 2024 3,200 1 View

I am doing Nano-IR technique, I need suggestion where I can find peaks for Aptamers?

I have grafting aptamers onto the polymer membrane surface. I found one peak near 1700cm for bases. what about other specific peaks?

25 March 2024 7,968 0 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close