Does anyone have experience in staining Bacillus spores for Flow cytometry?

06 July 2016 4,075 4 View

Can in vitro activation of PBMC with ionomycin and PMA for 4 hours in the presence of brefeldin induce this transient expression of Foxp3 in T cells?

Several studies have suggested that T-cell receptor (TCR) stimulation with anti-CD3 and anti-CD28 can induce FOXP3 expression in human CD4+CD25 -cells. Most of the papers stimulate the cells for...

02 March 2014 9,261 3 View

How do we carry out the statistical analysis for the quantification of atherosclerosis?

Murine atherosclerosis is induced by a high fat diet in susceptible mice with either APoE-/- or LDLr-/- in c57 background. Sections are taken from the aortic sinus to quantify area of plaque in...

01 February 2014 6,681 7 View

How to improve growth of MRC-5 Cells in vitro

I have been using MRC-5 fibroblast cells for in vitro assays. I am facing a problem with the growth of the cells now. They are very slow . I have tried changing media and its components,...

01 February 2013 6,323 0 View

Inducing oral tolerance to protein in rabbits.

How do you decide on the range of doses in rabbits? I know the dose in mice which gave us good tolerance. How is the dose escalation from mice to rabbits work? Especially to induce low dose oral...

04 May 2012 2,037 2 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

Experimental Design: How deal with pseudreplicates with immunological and clinical-chemical paramaeters?

Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...

24 February 2021 1,243 1 View

Looking for advices and tips to improve the staining and the gating strategy in human blood?

Hi, I am staining a human blood with CD19, CD3,CD14 and CD16. My gating strategy in FlowJo goes like this: - First, I gate a general population SSC-A VS FSC-A => Then gate for single cells, =>...

21 February 2021 5,724 4 View

Can you polarize Jurkat cells into different T helper subsets?

I am wondering if anyone has had success with in vitro polarization (via cytokines) of Jurkat cells. My goal is to understand the affect of a drug on Treg induction and differentiation. I...

12 January 2021 3,606 2 View

Can only the spike protein of the virus provide immunity?

Virology, microbiology, immunology, vaccine

21 December 2020 424 16 View

Can somebody recommend me a decent textbook to learn more about signaling pathways and their mechanistic principles in general?

I studied biochemistry and molecular biology in my bachelors and Immunology in my masters. I never came across a suitable course for general signaling pathways and principles. My interest is...

13 December 2020 5,690 5 View

Is it possible to extract DNA or RNA from paraffinized biopsies ?

My lab have some skin biopsies that were previously conserved in paraffine , and i wanted to know if it is possible to extract DNA or RNA from them.

06 December 2020 7,964 6 View

Could the desmin mutation in myofibrillar myopathy be caused by an infection?

A patient with desminopathy (mutation Thr341Pro DES in a heterozygous state) with the progression of the disease has a decrease in taste and smell, immunosuppression, and an increase in IgA in the...

02 November 2020 5,886 6 View

Whis HLA-A2 TAAs are best for DC pulsation and CD8+ cytotoxic T cell induction?

When looking to establish Ag-specific CD8 cytotoxic T cells with autologous dendritic cells, there are numerous tumor-associated Ags to choose from. Does anybody know which Ags give the best...

27 October 2020 2,068 2 View

Resuspending cell pellet after ficoll centrifugation?

In my current project I am planning to isolate mononuclear cells from peritoneal ascites of cancer patients using ficoll centrifugation. However, following isolation of the mononuclear cell layer,...

26 October 2020 5,966 4 View