Lakshmi Mundkur

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Questions related from Lakshmi Mundkur

Does anyone have experience in staining Bacillus spores for Flow cytometry?

We are trying to enumerate Bacillus spores by FCM. Known count of spore suspension is activated at 70 deg for 10 min followed by 45 deg cooling before staining. Counting by microbiological plate...

07 July 2016 4,118 4 View

Can in vitro activation of PBMC with ionomycin and PMA for 4 hours in the presence of brefeldin induce this transient expression of Foxp3 in T cells?

Several studies have suggested that T-cell receptor (TCR) stimulation with anti-CD3 and anti-CD28 can induce FOXP3 expression in human CD4+CD25 -cells. Most of the papers stimulate the cells for...

03 March 2014 9,304 3 View

How do we carry out the statistical analysis for the quantification of atherosclerosis?

Murine atherosclerosis is induced by a high fat diet in susceptible mice with either APoE-/- or LDLr-/- in c57 background. Sections are taken from the aortic sinus to quantify area of plaque in...

02 February 2014 6,724 7 View

How to improve growth of MRC-5 Cells in vitro

I have been using MRC-5 fibroblast cells for in vitro assays. I am facing a problem with the growth of the cells now. They are very slow . I have tried changing media and its components,...

02 February 2013 6,365 0 View

Inducing oral tolerance to protein in rabbits.

How do you decide on the range of doses in rabbits? I know the dose in mice which gave us good tolerance. How is the dose escalation from mice to rabbits work? Especially to induce low dose oral...

05 May 2012 2,084 2 View

Induction of mucosal tolerance to small proteins in rabbits

What is the basis of deciding the doses? how does one calculate the dose escalation from mice to rabbits for low dose tolerance induction?

05 May 2012 3,063 1 View