Does anyone have experience in staining Bacillus spores for Flow cytometry?

06 July 2016 4,075 4 View

Can in vitro activation of PBMC with ionomycin and PMA for 4 hours in the presence of brefeldin induce this transient expression of Foxp3 in T cells?

Several studies have suggested that T-cell receptor (TCR) stimulation with anti-CD3 and anti-CD28 can induce FOXP3 expression in human CD4+CD25 -cells. Most of the papers stimulate the cells for...

02 March 2014 9,261 3 View

How do we carry out the statistical analysis for the quantification of atherosclerosis?

Murine atherosclerosis is induced by a high fat diet in susceptible mice with either APoE-/- or LDLr-/- in c57 background. Sections are taken from the aortic sinus to quantify area of plaque in...

01 February 2014 6,681 7 View

How to improve growth of MRC-5 Cells in vitro

I have been using MRC-5 fibroblast cells for in vitro assays. I am facing a problem with the growth of the cells now. They are very slow . I have tried changing media and its components,...

01 February 2013 6,323 0 View

Induction of mucosal tolerance to small proteins in rabbits

What is the basis of deciding the doses? how does one calculate the dose escalation from mice to rabbits for low dose tolerance induction?

04 May 2012 3,027 1 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

Experimental Design: How deal with pseudreplicates with immunological and clinical-chemical paramaeters?

Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...

24 February 2021 1,243 1 View

Looking for advices and tips to improve the staining and the gating strategy in human blood?

Hi, I am staining a human blood with CD19, CD3,CD14 and CD16. My gating strategy in FlowJo goes like this: - First, I gate a general population SSC-A VS FSC-A => Then gate for single cells, =>...

21 February 2021 5,724 4 View

Can you polarize Jurkat cells into different T helper subsets?

I am wondering if anyone has had success with in vitro polarization (via cytokines) of Jurkat cells. My goal is to understand the affect of a drug on Treg induction and differentiation. I...

12 January 2021 3,606 2 View

Can only the spike protein of the virus provide immunity?

Virology, microbiology, immunology, vaccine

21 December 2020 424 16 View

Can somebody recommend me a decent textbook to learn more about signaling pathways and their mechanistic principles in general?

I studied biochemistry and molecular biology in my bachelors and Immunology in my masters. I never came across a suitable course for general signaling pathways and principles. My interest is...

13 December 2020 5,690 5 View

Is it possible to extract DNA or RNA from paraffinized biopsies ?

My lab have some skin biopsies that were previously conserved in paraffine , and i wanted to know if it is possible to extract DNA or RNA from them.

06 December 2020 7,964 6 View

Could the desmin mutation in myofibrillar myopathy be caused by an infection?

A patient with desminopathy (mutation Thr341Pro DES in a heterozygous state) with the progression of the disease has a decrease in taste and smell, immunosuppression, and an increase in IgA in the...

02 November 2020 5,886 6 View

Whis HLA-A2 TAAs are best for DC pulsation and CD8+ cytotoxic T cell induction?

When looking to establish Ag-specific CD8 cytotoxic T cells with autologous dendritic cells, there are numerous tumor-associated Ags to choose from. Does anybody know which Ags give the best...

27 October 2020 2,068 2 View

Resuspending cell pellet after ficoll centrifugation?

In my current project I am planning to isolate mononuclear cells from peritoneal ascites of cancer patients using ficoll centrifugation. However, following isolation of the mononuclear cell layer,...

26 October 2020 5,966 4 View