Hello. I want to induce EMT in breast cancer cell line (BT-549) using IL-1B. I need advice on the appropriate dose and timing of IL-1B treatment for EMT induction.
I did not use IL-1b to induce EMT. It has been reported that A549 cells would undergo EMT upon 48-hour IL-1β (1 ng/ml) exposure - E-Cad protein reduction was very clear after 3~7 days of IL-1b exposure. You might use TGF-b as a positive control. Good luck.