why do we add orthophosphoric acid along with salkowski reagent for indole acetic acid production test?
I feel that the practice in teacher education in my country is below the expected performance level due to very poor management system. Hope I will learn something from your experiences.
02 March 2021 1,516 4 View
I am working on the MIC determination of various antibacterial agents (both water-soluble and- insoluble) against S. aureus, MRSA, and S. pyogenes. Some guidelines recommend using round-bottomed...
17 February 2021 7,029 3 View
Hey I'm trying to measure the concentration of a protein I've purified and then labeled with Atto 647 flourescent dye. The protein has no tryptophans or tyrosines (although does have...
04 February 2021 4,482 4 View
As you all know, the 21st century skills require higher order thinking. So, do you recommend CBE as a means to realize such kinds of skills?
05 October 2020 7,684 12 View
I am working on portfolio optimization using lower partial moment of order 1, can someone help me how to implement LPM-1 in excel sheet using "tau" as my threshold value as 0.00% and order (n) as...
05 July 2020 2,510 1 View
How to enter the different fertilizer rates (including a control) in RevMan?
02 March 2020 5,309 2 View
I have carried out linear interpolation to fill missing monthly rain fall data, and carried out homogeneity test using standard homogeneity test for the annual and seasonal time series from the...
28 December 2019 758 3 View
That is why do i need to do SEM after i have done TEM?
16 June 2019 8,733 4 View
I am working on hydrogen evolution. In some of my samples grown/sputtered on nickel foam and carbon cloth substrate, I found a very high positive current in negative potential as shown in the...
07 June 2019 5,940 3 View
Usually amino compounds become yellow from colorless on storage due to air oxidation and some other reasons. I have a oppposite experience for a silicone -propyl amine polymer which was yellow...
12 May 2019 5,053 1 View
I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...
02 March 2021 4,443 2 View
Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...
01 March 2021 8,169 2 View
based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...
26 February 2021 6,799 3 View
Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...
25 February 2021 3,443 4 View
I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.
24 February 2021 1,633 4 View
Hi! I would want to know if I can use acetic acid with an ELITE WAX Perkin Elmer column for Gas Chromatography. This column has a polyethylene glycol phase, but I can't find the manual to check...
22 February 2021 730 2 View
I have a fixed sample and would like to use the murexide reaction to test for uric acid composition. I no longer have fresh samples
22 February 2021 1,328 1 View
I notice that upon washing and air-drying of my RGO/SPCE surface and characterizing again via DPV, the current response towards ferricyanide probe significantly decreases. My worry is that the...
22 February 2021 5,324 3 View
I have used Picral etchant (4.2 g Picric acid, 10 mL Acetic acid, 70 mL Ethanol, 10 mL distilled water) but it didn't work. Is there any alternative?
21 February 2021 2,558 5 View
My baseline increases without any explanation, it is only fixed by changing the solution B, will it be the column? Reagents? Bomb? Detector? Solution A: H3PO4 0.01% IN WATER Solution B: H3PO4...
21 February 2021 5,656 3 View