i have selected these antibiotics penicillin, tetracycline, chloramphenicol, rifampin, fluoroquinolone, metronidazole, isoniazid and trimethoprim for the MIC study. suggest me in what these each antibiotics can be completely dissolved.
hello Saranya Shivashankar,
Hope these will be useful
Tetracycline - 50% ethanol
Chloramphenicol - 70% ethanol
Penicillin - water
Rifampicin - 100% ethanol
Trimethoprim - Water
Regards
Hi,
I recommend you to follow the CLSI or EUCAST guidelines to select the best solvents and diluents for your antimicrobials.
CLSI:
-Antimicrobial agent: solvent - diluent.
-Penicillin: water.
-Tetracycline: water.
-Chloramphenicol: 95% ethanol - water.
-Rifampin: Methanol (maximum concentration = 640 ug/mL) - water (with string).
-Metronidazole: DMSO - water.
-Trimethoprim: 0.05 mol/L lactic or hydrochloric acid, 10% of final volume - water (may require heat).
Good luck!
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