In vitro drug testing using 3D spheroid model?

24 May 2017 1 2K Report

Hi,

I want to test the efficacy of our anticancer compounds in 3D spheroid cell culture. We are working on A549 lung cancer cells.

Can someone suggest a protocol, publication or some other reference for the same?

Mans Broekgaarden

Hi Pratik,

In case you're still looking for a method, please see our recent publication in scientific reports, for a relatively easy live/dead stain and subsequent image analysis workflow.

https://www.nature.com/articles/s41598-017-16622-9

Best of luck,

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