there´s a nice paper on scratch assays in nature protocols (liang 2007). another paper using a SA is in nature (electrical signals control wound healing...). best of luck

Simplest is Scratch assay........cost effective, quick exp to do............

http://www.molbiolcell.org/content/15/1/294.abstract

Please go to main article........u will find all info........., any doubts drop a comment..................

Best,

Vivek

Check this paper out:

http://jcs.biologists.org/content/120/5/876.full.pdf+html

there´s a nice paper on scratch assays in nature protocols (liang 2007). another paper using a SA is in nature (electrical signals control wound healing...). best of luck

Thank you all for quick response

Scratch assay is the quickest assay but you wrong into reproducibility issues. If you want repeatable measurements consider looking at the ECIS wounding assay (www.applied biophysic.com) which produces a 250 um size injury consistently.

It depends on the type of wound in consideration.

scratch assay is the best and simple as Sunkari has mentioned above. But all assays measure certain set of parameters, so u may have to combine it with other methods too. If u have no possibility for good microscopy, quantification could be problematic. We had developed a method to quantify, but that requires LiCOR Odyssey (http://www.ncbi.nlm.nih.gov/pubmed/19743408). The nature protocol article, which someone mentioned above is a good start, but U ll have to decide about a practical way for quantification available for u.

Other links:

http://www.ncbi.nlm.nih.gov/pubmed?term=16818469

http://www.ncbi.nlm.nih.gov/pubmed/15357872

http://www.ncbi.nlm.nih.gov/pubmed/14747654

Good Luck

Wound healing is a natural phenomenon, so let us talk about wound healing promoting activity. Well, If you are trying to find any in vitro assay for wound healing. I may say that it first of all, depends on the model you select. If you are in context of mimicking human wound, then rat is a probable model. For this you can carry out in vitro hydroxyproline content study as a part of wound healing promoting activity. Apart from this in vitro antioxidant assays may support this. Other than these you can also carry out matrix metalloprotease inhibition studies to support infected wound healing promoting activity.

Thanks so much

I am skeptical of in vitro data for wound healing because some wound interventions work more actively with the body than others. The results of an in vitro test will not show the true benefits of these interventions. For example, wound dressings that are activated by wound fluid, or that pull nutrients from the body into the wound bed, become static rather than dynamic when placed in a petri dish.

Use animal models. I know they cost more, but otherwise your results have no meaning.

Burd, et al., 2007 is an interesting case in point. In vivo results showed that silver, widely thought to be benign, was cytotoxic in wound beds.

Burd A, Kwok CH, Hung SC, Chan HS, Gu H, Lam WK, Huang L. A comparative study of the cytotoxicity of silver-based dressings in monolayer cell, tissue explant, and animal models. Wound Repair & Regeneration 2007 Jan-Feb;15(1):94-104.

Wound healing is a complex process that many compartment affected it . So use animal models. When you used in vitro models only you assay a restricted factors that affected this process instead some of this factors have activity when other factors are present.

Linda, the aim of the project is to identify suitable in vitro assays for many identified plant components(phytochemicals) with potentials wound healing ability. I accept your point on in vivo models being a better model but the basis for every drug discovery and development is the in vitro assay which I have researched and identified to be many. In vitro assays cant be meaningless for wound healing rather they guide a scientist. basic antioxidant assays, anti- inflammatory assays and cell proliferation assays is very important and unique in vitro assays for wound healing.

You may need to read in the types of cellular junctions, healing and diseases also you can look into the cell proliferation and cell signiling mechanism. some published data in the NCBI:

http://www.ncbi.nlm.nih.gov/pubmed/17406593

http://www.ncbi.nlm.nih.gov/pubmed/15797209

http://www.ncbi.nlm.nih.gov/pubmed/19743408

Good luck

Christine, I would love to talk to you more about your research. I just completed a literature review about wound healing in rural areas of tropical developing countries. There are many researchers gathering plants used by traditional healers and looking for phytochemicals in them that may be beneficial as commercial products. However, this research is not my focus. I am trying to obtain baseline data about wound management in villages. As you were gathering your lists of herbal remedies, did you ask the healers how and when they apply each plant? If so, you have important data that has never been published. Ethnobotanists publish lists and evaluate plant components, but they do not usually ask the healers how or why they use the plants. If you have this information, please contact me!

Christine, you are correct that in vitro research has its place in discovering potential beneficial substances. After the in vitro research, however, it is important to take the next step and verify the results in vivo.

According to recent literature- Manuka Honey is the best wound healer.

Manuka honey is produced in New Zealand by bees that pollinate the native manuka bush. Advocates say it treats wound infections and other conditions.

Healing Power of Honey

Honey has been used since ancient times to treat multiple conditions. It wasn't until the late 19th century that researchers discovered that honey has natural antibacterial qualities.

Honey protects against damage caused by bacteria. Some honey also stimulates production of special cells that can repair tissue damaged by infection. In addition, honey has an anti-inflammatory action that can quickly reduce pain and inflammation once it is applied.

But not all honey is the same. The antibacterial quality of honey depends on the type of honey as well as when and how it's harvested. Some kinds of honey may be 100 times more potent than others.

Components of Manuka Honey

Hydrogen peroxide is a component of honey. It gives most honey its antibiotic quality. But some types of honey, including manuka honey, also have other components with antibacterial qualities.

\The major antibacterial component in manuka honey is methylglyoxal (MG). MG is a compound found in most types of honey, but usually only in small quantities.

In manuka honey, MG comes from the conversion of another compound -- dihydroxyacetone -- that is found in high concentration in the nectar of manuka flowers. MG gives manuka honey its antibacterial power. The higher the concentration of MG, the stronger the antibiotic effect.

Honey producers have developed a scale for rating the potency of manuka honey. The rating is called UMF, which stands for Unique Manuka Factor.

The UMF rating corresponds with the concentration of MG. Not all honey labeled as manuka honey contains significant levels of MG. To be considered potent enough to be therapeutic, manuka honey needs a minimum rating of 10 UMF. Honey at or above that level is marketed as "UMF Manuka Honey" or "Active Manuka Honey."

How Manuka Honey Is Used:

The main medical use for manuka honey is on top of a wound. It is generally used for treating minor wounds and burns.

Manuka honey is also marketed for use in many other conditions. These include:

Preventing and treating cancer

Reducing high cholesterol

Reducing systemic inflammation

Treating diabetes

Treating eye, ear, and sinus infections

Treating gastrointestinal problems

But the evidence is limited on whether or not manuka honey is effective for these conditions.

The honey used to treat wounds is a medical-grade honey. It is specially sterilized and prepared as a dressing. So the jar of manuka honey in the pantry should not be considered part of a first aid kit. Wounds and infections should be seen and treated by a health care professional.

What the Science Says About Manuka Honey

Several recent studies show manuka honey is effective when used on top of wounds and leg ulcers. Studies also show it's effective in fighting infection and promoting healing.

But not all studies show that it helps to heal ulcers. And there is concern that manuka honey may actually delay healing in people who have ulcers related to diabetes.

The Natural Medicines Comprehensive Database lists honey as being "possibly effective" to treat burns and wounds. The Cochrane Review notes that honey may shorten healing times in mild burns compared with traditional dressings. However, honey dressings do not increase leg ulcer healing at 12 weeks even when used with compression wraps.

Another recent study suggests that manuka honey may be effective in preventing gingivitis and other periodontal disease by reducing the buildup of plaque. And in 2010, the scientific steering committee of the National Cancer Institute approved a proposal for the use of manuka honey for the reduction of inflammation of the esophagus associated with chemotherapy.

Another possible benefit of honey is that, unlike antibiotics, it has not been reported to cause development of resistant bacteria. These so-called "superbugs" develop after repeated exposure to common antibiotics. They require special antibiotics to treat them.

So far, studies have not shown manuka honey to be effective for treating high cholesterol or balancing the bacteria in the gastrointestinal tract.

Also, no major studies have looked at the effect of manuka honey on cancer, diabetes, or fungal infections.

Possible Side Effects of Manuka Honey

The possible side effects of manuka honey are:

Allergic reaction, especially in people who are allergic to bees

Risk of a rise in blood sugar

Possible interaction with certain chemotherapy drugs

Most of the studies on manuka honey have been with small numbers of patients. More studies are needed to decide if it is safe and effective for various medical conditions.

You sounds like a sales rep. Only Manuka Honey and not other honey? Sounds suspicious to me. While there are some honeys that are too watery to have healing properties, Manuka Honey is not the only honey that does.

You may be correct, as the information I gathered was from webmd.com, which claims to be the leading source for trustworthy and timely health and medical news and information. It is good if some watery honeys are also effective. Thanks for information.

Go to http://www.gredeco.com/gredeco-en.htm

They have developed an exclusive method of experimentation which answers your question... They are good friends of mine and I'm currently working on Low Level Laser wound healing....

My site (sorry it's in french) concerning my private practice :

http://www.phototherapies.com

I have gone through your site (in French) and could not understand it. Will you please send me a summary of it in English!!

In vitro hydroxyproline assay can support wound healing model. and Zymogram gel prolfe can support infected wound healing model.

Easiest and most straight forward assay..... grow adherent cells eg HeLa cells, once confluent, use a sterile pippette to scratch a cross sign on the cells and check your plates next day. ofcourse you will have controls for your work e.g for a tumour suppressor assay, you have a plate with the gene of interest i.e TSG and on your control, you have vector only.......... you expect the wound not to heal in the presence of TSG as it will exhibit anti-proliferative effect.........

Nice monday scientists!

I was wondering if anybody could give me a useful trick for making the scratch on each well / dish more constant in terms of the width of the gap in the scratch wound assay?

It is tough even though some people manage it (it also depends on your cells). After some unsuccesful trials we finally standardized a different method making uniform scratching unnecessary (PMID 19743408). 

file:///C:/Users/heidari/Downloads/974580.pdf

Do we need to coat the dish or plate with ECM before migration assay?