Vivekananda Gupta Sunkari

4 Questions 15 Answers 0 Followers

Questions related from Vivekananda Gupta Sunkari

Cholera toxin in cell culture?

Hello Scientists, I am planning to culture prostate cancer cell line and it requires addition of Cholera toxin, I am trying to find a proper way of disposal of the cell culture media and the...

08 August 2018 9,367 3 View

Insulin staining plus additional staining?

Hello Everyone, I am staining for Insulin in treated and untreated mice, should I stain for any other things - like Glucagon etc. Would Insulin is sufficient? Please advise on this.

01 January 2016 1,112 4 View

Can anyone suggest good FoxP3 and CD4 immuno histo stainings, antibodies and isotype controls?

I want to stain for sections in Spleen, Pancreas and Skin.

04 April 2014 5,443 4 View

Stable cell line generation and antibiotic resistance

Subject: Stable Transfection Trouble Shooting I am electroporating several plasmids into an already CRISPR modified Cancer Cell line. All the cells look fine post electroporation. However, once I...

01 January 1970 2,747 4 View