In the preparation of 1X TE buffer, is there any difference in the prepared buffered between Tris-HCl and Tris base?

09 September 2013 0 6K Report

Will the use of Tris-HCl or Tris base have any effect on the prepared 1X TE buffer?

Badges
Science topic

More Teck Nam Ang's questions See All

Can I use LAMMPS molecular dynamics simulator for adsorption of VOC to activated carbons?

I was searching for an open source molecular dynamics simulation software to start with, and I found LAMMPS. I am a newbie in this field. Could you give me some advices.

05 June 2019 6,689 3 View

For enzyme inactivation, is it necessary to adjust the pH of sample to slightly acidic (~3.8) before boiling it?

This protocol is reported in a journal written by Paolucci-Jeanjean et al. (2000).

07 August 2013 1,049 25 View

Very low A260/A230 ratio in extracted DNA from sludge sample using FastDNA SPIN kit for soil.

I am using FastDNA SPIN kit for soil to extract DNA from sludge samples. However, the A260/A230 ratio is very low (i.e. 0.2), which signifies contamination of humic substance. Does anyone use the...

03 April 2013 5,704 6 View

In designing an optimization experiment, can we consider duration as one of the parameters? (The experiment will be carried out in batch.)

Optimization using response surface methodology.

06 July 2012 9,337 16 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View